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DAAM1 and PREP are involved in human spermatogenesis
- Publication Year :
- 2020
-
Abstract
- During differentiation of the male gamete, there is a massive remodelling in the shape and architecture of all the cells in the seminiferous epithelium. The cytoskeleton, as well as many associated proteins, plays a pivotal role in this process. To better characterise the factors involved, we analysed two proteins: the formin, dishevelled-associated activator of morphogenesis 1 (DAAM1), which participates in the regulation of actin polymerisation, and the protease, prolyl endopeptidase (PREP), engaged in microtubule-associated processes. In our previous studies we demonstrated their involvement in cytoskeletal dynamics necessary for correct postnatal development of the rat testis. Here, we used samples of testicular tissue obtained from infertile men by testicular sperm extraction and the spermatozoa of asthenoteratozoospermic patients. By western blot and immunofluorescent analysis, we found that DAAM1 and PREP expression and localisation were impaired in both the testis and spermatozoa, and in particular in the midpiece as well as in the principal and end-pieces of the flagella, as compared with spermatozoa of normospermic men. Our results provide new knowledge of the dynamics of spermatogenesis, raising the possibility of using DAAM1 and PREP as new markers of normal fertility.
- Subjects :
- Adult
Male
rho GTP-Binding Proteins
Reproductive technology
Mitochondrial Proteins
03 medical and health sciences
0302 clinical medicine
Endocrinology
Prolyl endopeptidase
Testis
Genetics
medicine
Humans
Spermatogenesis
Cytoskeleton
Molecular Biology
030304 developmental biology
0303 health sciences
DAAM1
030219 obstetrics & reproductive medicine
Sperm Count
biology
DAAM!, PREP, Fertility, Cytoskeleton, Testis, Spermatozoa, Human
Microfilament Proteins
Serine Endopeptidases
Spermatozoa
Testicular sperm extraction
Cell biology
medicine.anatomical_structure
Reproductive Medicine
Asthenozoospermia
Case-Control Studies
Formins
Sperm Motility
biology.protein
Gamete
Animal Science and Zoology
Developmental Biology
Biotechnology
medicine.drug
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....19aa4278fc2d7b7947496398349243bf