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Photometric procedure for quantitative analysis of Aflatoxin B1 in peanuts by thin-layer chromatography using charge coupled device detector
- Source :
- Química Nova v.33 n.1 2010, Química Nova, Sociedade Brasileira de Química (SBQ), instacron:SBQ, Química Nova, Volume: 33, Issue: 1, Pages: 43-47, Published: 2010, Repositório Institucional da UFRGS, Universidade Federal do Rio Grande do Sul (UFRGS), instacron:UFRGS, Química Nova, Vol 33, Iss 1, Pp 43-47 (2010)
- Publication Year :
- 2010
- Publisher :
- Sociedade Brasileira de Química, 2010.
-
Abstract
- A photometric procedure was developed for determination of aflatoxin B1 in peanuts by TLC-CCD technique. The quantification and detection limit were 1.2 μg kg-1 and 0.4 ng per spot, respectively, with mean recovery of 98%. The CCD camera is sufficiently sensitive to detect small changes in spots fluorescence intensity and the results for performance confirmed the efficiency of the method. Another important property of CCD detector is its linearity for a wide range of luminous stimulus determined by analysis of five-point calibration curves using the intensity of AFB1 fluorescence versus AFB1 concentration (0.8 to 4.8 ng per spot). The method was applied to the analysis of thirty nine peanut samples and aflatoxin B1 levels ranged from 16 to 115 μg kg-1. The TLC-CCD and the photometric procedure developed in this study demonstrated to be a simple and efficient tool for quantitative analyses of AFB1 in peanut samples.
- Subjects :
- Detection limit
Aflatoxin
Chromatography
Spots
Chemistry
Calibration curve
Detector
Analytical chemistry
photometric procedure
Aflatoxina [Amendoim]
General Chemistry
TLC-CCD
Fluorescence
Thin-layer chromatography
lcsh:Chemistry
lcsh:QD1-999
Amendoim
aflatoxin B1
Charge-coupled device
Aflatoxina B1
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Química Nova v.33 n.1 2010, Química Nova, Sociedade Brasileira de Química (SBQ), instacron:SBQ, Química Nova, Volume: 33, Issue: 1, Pages: 43-47, Published: 2010, Repositório Institucional da UFRGS, Universidade Federal do Rio Grande do Sul (UFRGS), instacron:UFRGS, Química Nova, Vol 33, Iss 1, Pp 43-47 (2010)
- Accession number :
- edsair.doi.dedup.....19a006ff6dcb2bd909016711583feba1