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Detection and characterization of pangola stunt Fijivirus from Australia using cloned cDNA probes
- Source :
- Archives of Virology. 135:397-404
- Publication Year :
- 1994
- Publisher :
- Springer Science and Business Media LLC, 1994.
-
Abstract
- Four cDNA clones were generated from the genomic dsRNA of an Australian isolate of pangola stunt Fijivirus (PaSV). Each clone hybridized with nucleic acid extracts from PaSV infected plants but not healthy plants. Further, each clone hybridized with more than one segment of the PaSV dsRNA genome. One clone was used to demonstrate that homology existed between the Australian isolate of PaSV and a South American isolate of PaSV although the isolates differed in the sizes of the genomic dsRNAs and in the vector species. The clone also hybridized with some segments of the maize rough dwarf Fijivirus genome.
- Subjects :
- DNA, Complementary
Genome, Viral
Biology
Poaceae
Reoviridae
Genome
Homology (biology)
Sequence Homology, Nucleic Acid
Virology
Complementary DNA
Plant virus
Cloning, Molecular
RNA, Double-Stranded
Genetics
Hybridization probe
Australia
food and beverages
Fijivirus
General Medicine
South America
biology.organism_classification
Deoxycytosine Nucleotides
Nucleic acid
Autoradiography
DNA Probes
Molecular probe
Phosphorus Radioisotopes
Subjects
Details
- ISSN :
- 14328798 and 03048608
- Volume :
- 135
- Database :
- OpenAIRE
- Journal :
- Archives of Virology
- Accession number :
- edsair.doi.dedup.....199d34515fe433d4d5e28d4b1bf7dae3