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Analysis of gap junctional intercellular communications using a dielectrophoresis-based microchip
- Source :
- European Journal of Cell Biology, European Journal of Cell Biology, 2017, [Epub ahead of print]. ⟨10.1016/j.ejcb.2017.01.003⟩, European Journal of Cell Biology, Elsevier, 2017, [Epub ahead of print]. ⟨10.1016/j.ejcb.2017.01.003⟩
- Publication Year :
- 2017
- Publisher :
- HAL CCSD, 2017.
-
Abstract
- International audience; Please cite this article in press as: Tellez-Gabriel, M., et al., Analysis of gap junctional intercellular communications using a dielectrophoresis-based microchip. Gap junctions are transmembrane structures that directly connect the cytoplasm of adjacent cells, making intercellular communications possible. It has been shown that the behaviour of several tumours – such as bone tumours – is related to gap junction intercellular communications (GJIC). Several methodologies are available for studying GJIC, based on measuring different parameters that are useful for multiple applications, such as the study of carcinogenesis for example. These methods nevertheless have several limitations. The present manuscript describes the setting up of a dielectrophoresis (DEP)-based lab-on-a-chip platform for the real-time study of Gap Junctional Intercellular Communication between osteosarcoma cells and the main cells accessible to their microenvironment. We conclude that using the DEParray technology for the GJIC assessment has several advantages comparing to current techniques. This methodology is less harmful for cells integrity; cells can be recovered after interaction to make further molecular analysis; it is possible to study GJIC in real time; we can promote cell interactions using up to five different populations. The setting up of this new methodology overcomes several difficulties to perform experiments for solving questions about GJIC process that we are not able to do with current technics.
- Subjects :
- 0301 basic medicine
Histology
Bone microenvironment
Computer science
Bone Neoplasms
[SDV.CAN]Life Sciences [q-bio]/Cancer
Cell Communication
Time-Lapse Imaging
Pathology and Forensic Medicine
Electrophoresis, Microchip
03 medical and health sciences
0302 clinical medicine
[SDV.MHEP.PED] Life Sciences [q-bio]/Human health and pathology/Pediatrics
[SDV.CAN] Life Sciences [q-bio]/Cancer
Bone tumours
Cell Line, Tumor
Humans
[SDV.MHEP.EM] Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism
Osteosarcoma
[SDV.MHEP.RSOA] Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system
[SDV.MHEP.PED]Life Sciences [q-bio]/Human health and pathology/Pediatrics
Osteoblasts
Gap junction
Multiple applications
Gap Junctions
Cell Differentiation
Mesenchymal Stem Cells
Gap junctional intercellular communication
Cell Biology
General Medicine
Dielectrophoresis
[SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism
Molecular analysis
Cell biology
030104 developmental biology
[SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system
030220 oncology & carcinogenesis
Dielectrophoresis device
Intracellular
Subjects
Details
- Language :
- English
- ISSN :
- 01719335
- Database :
- OpenAIRE
- Journal :
- European Journal of Cell Biology, European Journal of Cell Biology, 2017, [Epub ahead of print]. ⟨10.1016/j.ejcb.2017.01.003⟩, European Journal of Cell Biology, Elsevier, 2017, [Epub ahead of print]. ⟨10.1016/j.ejcb.2017.01.003⟩
- Accession number :
- edsair.doi.dedup.....197f6856a4c30c6b87a74c9699f56feb