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Modulation of lysine methylation in myocyte enhancer factor 2 during skeletal muscle cell differentiation
- Source :
- NUCLEIC ACIDS RESEARCH(42): 1, Nucleic Acids Research
- Publication Year :
- 2013
- Publisher :
- Oxford University Press (OUP), 2013.
-
Abstract
- Myocyte enhancer factor 2 (MEF2) is a family of transcription factors that regulates many processes, including muscle differentiation. Due to its many target genes, MEF2D requires tight regulation of transcription activity over time and by location. Epigenetic modifiers have been suggested to regulate MEF2-dependent transcription via modifications to histones and MEF2. However, the modulation of MEF2 activity by lysine methylation, an important posttranslational modification that alters the activities of transcription factors, has not been studied. We report the reversible lysine methylation of MEF2D by G9a and LSD1 as a regulatory mechanism of MEF2D activity and skeletal muscle differentiation. G9a methylates lysine-267 of MEF2D and represses its transcriptional activity, but LSD1 counteracts it. This residue is highly conserved between MEF2 members in mammals. During myogenic differentiation of C2C12 mouse skeletal muscle cells, the methylation of MEF2D by G9a decreased, on which MEF2D-dependent myogenic genes were upregulated. We have also identified lysine-267 as a methylation/demethylation site and demonstrate that the lysine methylation state of MEF2D regulates its transcriptional activity and skeletal muscle cell differentiation.
- Subjects :
- Mef2
animal structures
Transcription, Genetic
Myoblasts, Skeletal
Gene Regulation, Chromatin and Epigenetics
Biology
Methylation
Cell Line
Mice
Skeletal muscle cell differentiation
Epigenetics of physical exercise
Histone methylation
Genetics
Animals
Humans
Histone Demethylases
MEF2 Transcription Factors
Lysine
EZH2
Pioneer factor
Cell Differentiation
Oxidoreductases, N-Demethylating
Histone-Lysine N-Methyltransferase
musculoskeletal system
Chromatin
HEK293 Cells
Biochemistry
Histone methyltransferase
Protein Processing, Post-Translational
Subjects
Details
- ISSN :
- 13624962 and 03051048
- Volume :
- 42
- Database :
- OpenAIRE
- Journal :
- Nucleic Acids Research
- Accession number :
- edsair.doi.dedup.....197b3987727a261e8a611b997121da21