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Matrix-assisted laser desorption/ionization time of flight mass spectrometry identification of Vibrio (Listonella) anguillarum isolated from sea bass and sea bream
- Source :
- PLoS ONE, PLOS ONE, PLoS ONE, Vol 14, Iss 11, p e0225343 (2019)
- Publication Year :
- 2019
- Publisher :
- Public Library of Science, 2019.
-
Abstract
- Vibrio(Listonella)anguillarumis a pathogenic bacterium causing septicaemia in a wide range of marine organisms and inducing severe mortalities, thus it is crucial to conduct its accurate and rapid identification. The aim of this study was to assess MALDI-TOF MS as a method of choice for identification of clinicalV.anguillarumisolates from affected marine fish. Since the method accuracy might be influenced by the type of the medium used, as well as by the incubation conditions, we testedV.anguillarumisolates grown on standard media with and without the addition of NaCl, cultured at three incubation temperatures, and at three incubation periods. The best scores were retrieved forV.anguillarumstrains grown on NaCl-supplemented tryptone soy agar (TSA) at 22°C and incubated for 48h (100% identification to species level; overall score 2.232), followed by incubation at 37°C and 48h (100% to species level; score 2.192). The strains grown on non-supplemented TSA gave the best readings when incubated at 22°C for 72h (100% identification to species level; overall score 2.182), followed by incubation at 15°C for 72h (100% to species level; score 2.160). Unreliable identifications and no-identifications were growing with the incubation duration at 37°C, on both media, amounting to 88.89% for 7d incubation on supplemented TSA, and 92.60% for 7d incubation on non-supplemented TSA. The age of the cultured strains and use of media significantly impacted the mass spectra, demonstrating that for reliable identification, MALDI-TOF MS protein fingerprinting with the on-target extraction should be performed on strains grown on a NaCl-supplemented medium at temperatures between 15 and 22°C, incubated for 48–72 hours.
- Subjects :
- 0301 basic medicine
Molecular biology
DNA hybridization
Marine and Aquatic Sciences
Pathology and Laboratory Medicine
Listonella anguillarum
Mass Spectrometry
Analytical Chemistry
chemistry.chemical_compound
Spectrum Analysis Techniques
Peptide mass fingerprinting
Medicine and Health Sciences
Agar
Cluster Analysis
Marine Fish
Food science
Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry
Incubation
Listonella
Multidisciplinary
biology
Chemistry
MALDI-TOF MS
Vibrio anguillarum
Eukaryota
Mass Spectra
sea ba
Marine Bacteria
Bacterial Pathogens
Medical Microbiology
Physical Sciences
Vertebrates
Medicine
Pathogens
Research Article
Veterinary Medicine
food.ingredient
Science
030106 microbiology
Marine Biology
Research and Analysis Methods
Microbiology
03 medical and health sciences
food
Signs and Symptoms
Diagnostic Medicine
Animals
14. Life underwater
Sea bass
Microbial Pathogens
Vibrio
Vibrio (Listonella) anguillarum
Molecular probe techniques
Bacteria
Organisms
Biology and Life Sciences
Septicemia
biology.organism_classification
Probe hybridization
030104 developmental biology
Fish
Molecular biology techniques
Tryptone
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Earth Sciences
Bass
sea bream
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- PLoS ONE, PLOS ONE, PLoS ONE, Vol 14, Iss 11, p e0225343 (2019)
- Accession number :
- edsair.doi.dedup.....19756067820f2d523592df5002a66ca7