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Untargeted and targeted gingival metabolome in rodents reveal metabolic links between high-fat diet-induced obesity and periodontitis
- Source :
- Journal of clinical periodontologyREFERENCES. 48(8)
- Publication Year :
- 2021
-
Abstract
- Aim To characterize gingival metabolome in high-fat diet (HFD)-induced obesity in mice with/without periodontitis. Methods HFD-induced obesity mouse model was established by 16-week feeding, and a lean control group was fed with low-fat diet (n = 21/group). Both models were induced for periodontitis on the left sides by molar ligation for 10 days, whereas the right sides were used as controls. Gingival metabolome and arginine metabolism were analysed by non-targeted/targeted liquid chromatography-mass spectrometry. Results Of 2247 reference features, presence of periodontitis altered 165 in lean versus 885 in HFD mice; and HFD altered 525 in absence versus 1435 in presence of periodontitis. Compared with healthy condition, periodontitis and HFD had distinct effects on gingival metabolome. Metabolomic impacts of periodontitis were generally greater in HFD mice versus lean controls. K-medoids clustering showed that HFD amplified the impacts of periodontitis on gingival metabolome in both intensity and extensity. Ten metabolic pathways were enriched, including 2 specific to periodontitis, 5 specific to HFD and 3 shared ones. Targeted validation on arginine metabolism confirmed the additive effects between HFD and periodontitis. Conclusion The obese population consuming excessive HFD display amplified metabolic response to periodontitis, presenting a metabolic susceptibility to exacerbated periodontal destruction.
- Subjects :
- Lean control
medicine.medical_specialty
Population
Rodentia
Diet, High-Fat
03 medical and health sciences
High fat diet induced obesity
Mice
0302 clinical medicine
Metabolomics
Internal medicine
Arginine metabolism
medicine
Metabolome
Animals
030212 general & internal medicine
Obesity
education
Periodontitis
education.field_of_study
business.industry
digestive, oral, and skin physiology
food and beverages
nutritional and metabolic diseases
030206 dentistry
medicine.disease
Mice, Inbred C57BL
Endocrinology
Periodontics
business
Subjects
Details
- ISSN :
- 1600051X
- Volume :
- 48
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Journal of clinical periodontologyREFERENCES
- Accession number :
- edsair.doi.dedup.....19683704f6f071a345ba657f37a8ae25