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RNA Sequence and Base Pairing Effects on Insertion Editing in Trypanosoma brucei
- Source :
- Molecular and Cellular Biology. 22:1567-1576
- Publication Year :
- 2002
- Publisher :
- Informa UK Limited, 2002.
-
Abstract
- RNA editing inserts and deletes uridylates (U's) in kinetoplastid mitochondrial pre-mRNAs by a series of enzymatic steps. Small guide RNAs (gRNAs) specify the edited sequence. Editing, though sometimes extensive, is precise. The effects of mutating pre-mRNA and gRNA sequences in, around, and upstream of the editing site on the specificity and efficiency of in vitro insertion editing were examined. U's could be added opposite guiding pyrimidines, but guiding purines, particularly A's, were required for efficient ligation. A base pair between mRNA and gRNA immediately upstream of the editing site was not required for insertion editing, although it greatly enhanced its efficiency and accuracy. In addition, a gRNA/mRNA duplex upstream of the editing site enhanced insertion editing when it was close to the editing site, but prevented cleavage, and hence editing, when immediately adjacent to the editing site. Thus, several aspects of mRNA-gRNA interaction, as well as gRNA base pairing with added U's, optimize editing efficiency, although they are not required for insertion editing.
- Subjects :
- Genetics
Messenger RNA
RNA Stability
RNA, Mitochondrial
Base pair
Trypanosoma brucei brucei
Gene Expression
RNA
Cell Biology
Computational biology
Biology
Cleavage (embryo)
Duplex (building)
RNA editing
Animals
Nucleic Acid Conformation
RNA Editing
Guide RNA
Uridine Monophosphate
Base Pairing
Molecular Biology
RNA, Protozoan
RNA, Guide, Kinetoplastida
Subjects
Details
- ISSN :
- 10985549
- Volume :
- 22
- Database :
- OpenAIRE
- Journal :
- Molecular and Cellular Biology
- Accession number :
- edsair.doi.dedup.....1965e3cf6dbe6fe6ceeef09604593a62