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A new amplification strategy for ultrasensitive electrochemical aptasensor with network-like thiocyanuric acid/gold nanoparticles

Authors :
Guifang Cheng
Jing Zheng
Wanjuan Feng
Fan Zhang
Yuzhi Fang
Pingang He
Li Lin
Source :
Biosensorsbioelectronics. 23(3)
Publication Year :
2007

Abstract

An ultrasensitive and highly specific electrochemical aptasensor for detection of thrombin based on gold nanoparticles and thiocyanuric acid is presented. For this proposed aptasensor, aptamerI was immobilized on the magnetic nanoparticles, aptamerII was labeled with gold nanoparticles. The magnetic nanoparticle was used for separation and collection, and gold nanoparticle offered excellent electrochemical signal transduction. Through the specific recognition for thrombin, a sandwich format of magnetic nanoparticle/thrombin/gold nanoparticle was fabricated, and the signal amplification was further implemented by forming network-like thiocyanuric acid/gold nanoparticles. A significant sensitivity enhancement had been obtained, and the detection limit was down to 7.82 aM. The presence of other proteins such as BSA and lysozyme did not affect the detection of thrombin, which indicates a high specificity of thrombin detection could be achieved. This electrochemical aptasensor is expected to have wide applications in protein monitoring and disease diagnosis.

Details

ISSN :
09565663
Volume :
23
Issue :
3
Database :
OpenAIRE
Journal :
Biosensorsbioelectronics
Accession number :
edsair.doi.dedup.....1954ecafa4902f109606172c39d9f3e0