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An overview of using fungal DNA for the diagnosis of invasive mycoses
- Source :
- Expert Review of Molecular Diagnostics, Expert Review of Molecular Diagnostics, 2022, 22 (2), pp.169-184. ⟨10.1080/14737159.2022.2037423⟩, White, P L, Alanio, A, Brown, L, Cruciani, M, Hagen, F, Gorton, R, Lackner, M, Millon, L, Morton, C O, Rautemaa-richardson, R, Barnes, R A, Donnelly, J P & Loffler, J 2022, ' An overview of using fungal DNA for the diagnosis of invasive mycoses ', Expert Review of Molecular Diagnostics . https://doi.org/10.1080/14737159.2022.2037423
- Publication Year :
- 2022
-
Abstract
- Introduction: Fungal PCR has undergone considerable standardization and together with the availability of commercial assays, external quality assessment schemes and extensive performance validation data, is ready for widespread use for the screening and diagnosis of invasive fungal disease (IFD).Areas Covered: Drawing on the experience and knowledge of the leads of the various working parties of the Fungal PCR initiative, this review will address general considerations concerning the use of molecular tests for the diagnosis of IFD, before focussing specifically on the technical and clinical aspects of molecular testing for the main causes of IFD and recent technological developments.Expert Opinion: For infections caused by Aspergillus, Candida and Pneumocystis jirovecii, PCR testing is recommended, combination with serological testing will likely enhance the diagnosis of these diseases. For other IFD (e.g. Mucormycosis) molecular diagnostics, represent the only non-classical mycological approach towards diagnoses and continued performance validation and standardization has improved confidence in such testing. The emergence of antifungal resistance can be diagnosed, in part, through molecular testing. Next-generation sequencing has the potential to significantly improve our understanding of fungal phylogeny, epidemiology, pathogenesis, mycobiome/microbiome and interactions with the host, while identifying novel and existing mechanisms of antifungal resistance and novel diagnostic/therapeutic targets.
- Subjects :
- MESH: Fungi
mucorales PCR
MESH: Molecular Diagnostic Techniques
Polymerase Chain Reaction
Pathology and Forensic Medicine
Genetics
Humans
candida PCR
Fungi/genetics
DNA, Fungal
Molecular Biology
aspergillus PCR
[SDV.EE.SANT]Life Sciences [q-bio]/Ecology, environment/Health
MESH: Humans
Fungal/genetics
PCP PCR
Fungi
MESH: Polymerase Chain Reaction
DNA
fungal PCR
MESH: DNA, Fungal
Molecular Diagnostic Techniques
Molecular Medicine
Invasive fungal disease
Invasive Fungal Infections/diagnosis
Invasive Fungal Infections
MESH: Invasive Fungal Infections
Subjects
Details
- Language :
- English
- ISSN :
- 14737159 and 17448352
- Volume :
- 22
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Expert review of molecular diagnostics
- Accession number :
- edsair.doi.dedup.....194c898c3ee5b7a6b094cd2497349088