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Effect of curcumin on glycerol-induced acute kidney injury in rats
- Source :
- Scientific Reports, Vol 7, Iss 1, Pp 1-11 (2017), Scientific Reports
- Publication Year :
- 2017
- Publisher :
- Nature Publishing Group, 2017.
-
Abstract
- The aim of this study was to investigate the protective role and underlying mechanisms of curcumin on glycerol-induced acute kidney injury (AKI) in rats. Glycerol (10 ml/kg BW, 50% v/v in sterile saline, i.m.) was used to induce AKI, followed by curcumin (200 mg/kg/day, p.o.) administration for 3 days. To confirm renal damage and the effects of curcumin on AKI, serum BUN, Scr, and CK as well as renal SOD, MDA, GSH-Px were measured. Additionally, morphological changes were identified by H&E staining and transmission electron microscopy. The expression of several factors including chemotactic factor MCP-1, proinflammatory cytokines including TNF-α and IL-6, as well as the kidney injury markers, as Kim-1 and Lipocalin-2 were also assessed using q-PCR. Finally, cell apoptosis in renal tissue was detected using in situ TUNEL apoptosis fluorescence staining and expression of proteins associated with apoptotic, oxidative stress and lipid oxidative related signaling pathways were detected using immunohistochemical staining and western blot. The results showed that curcumin exerts renoprotective effects by inhibiting oxidative stress in rhabdomyolysis-induced AKI through regulation of the AMPK and Nrf2/HO-1 signaling pathways, and also ameliorated RM-associated renal injury and cell apoptosis by activating the PI3K/Akt pathway.
- Subjects :
- Glycerol
0301 basic medicine
Pathology
medicine.medical_specialty
Curcumin
NF-E2-Related Factor 2
lcsh:Medicine
Apoptosis
Pharmacology
Kidney
medicine.disease_cause
urologic and male genital diseases
Article
Antioxidants
Proinflammatory cytokine
Rats, Sprague-Dawley
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
AMP-Activated Protein Kinase Kinases
Lipocalin-2
Western blot
medicine
Animals
lcsh:Science
PI3K/AKT/mTOR pathway
Multidisciplinary
TUNEL assay
medicine.diagnostic_test
business.industry
Anti-Inflammatory Agents, Non-Steroidal
lcsh:R
Acute kidney injury
Acute Kidney Injury
medicine.disease
Rats
Oxidative Stress
030104 developmental biology
chemistry
030220 oncology & carcinogenesis
Female
lcsh:Q
business
Cell Adhesion Molecules
Protein Kinases
Heme Oxygenase-1
Oxidative stress
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 7
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....1929f760d5a47f39009321636b4eedf7
- Full Text :
- https://doi.org/10.1038/s41598-017-10693-4