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Effect of curcumin on glycerol-induced acute kidney injury in rats

Authors :
Dan Bao
Yuan Yin
Jindao Wu
Qingting Hao
Qin Chen
Xiongxiong Pan
Heling Fu
Daorong Hou
Youjin Dai
Yuan Zheng
Source :
Scientific Reports, Vol 7, Iss 1, Pp 1-11 (2017), Scientific Reports
Publication Year :
2017
Publisher :
Nature Publishing Group, 2017.

Abstract

The aim of this study was to investigate the protective role and underlying mechanisms of curcumin on glycerol-induced acute kidney injury (AKI) in rats. Glycerol (10 ml/kg BW, 50% v/v in sterile saline, i.m.) was used to induce AKI, followed by curcumin (200 mg/kg/day, p.o.) administration for 3 days. To confirm renal damage and the effects of curcumin on AKI, serum BUN, Scr, and CK as well as renal SOD, MDA, GSH-Px were measured. Additionally, morphological changes were identified by H&E staining and transmission electron microscopy. The expression of several factors including chemotactic factor MCP-1, proinflammatory cytokines including TNF-α and IL-6, as well as the kidney injury markers, as Kim-1 and Lipocalin-2 were also assessed using q-PCR. Finally, cell apoptosis in renal tissue was detected using in situ TUNEL apoptosis fluorescence staining and expression of proteins associated with apoptotic, oxidative stress and lipid oxidative related signaling pathways were detected using immunohistochemical staining and western blot. The results showed that curcumin exerts renoprotective effects by inhibiting oxidative stress in rhabdomyolysis-induced AKI through regulation of the AMPK and Nrf2/HO-1 signaling pathways, and also ameliorated RM-associated renal injury and cell apoptosis by activating the PI3K/Akt pathway.

Details

Language :
English
ISSN :
20452322
Volume :
7
Issue :
1
Database :
OpenAIRE
Journal :
Scientific Reports
Accession number :
edsair.doi.dedup.....1929f760d5a47f39009321636b4eedf7
Full Text :
https://doi.org/10.1038/s41598-017-10693-4