Back to Search
Start Over
Down selecting adjuvanted vaccine formulations: a comparative method for harmonized evaluation
- Source :
- BMC Immunology, Vol 19, Iss 1, Pp 1-11 (2018), BMC Immunology, BMC immunology, vol. 19, no. 1, pp. 6
- Publication Year :
- 2018
- Publisher :
- BMC, 2018.
-
Abstract
- Background: The need for rapid and accurate comparison of panels of adjuvanted vaccine formulations and subsequent rational down selection, presents several challenges for modern vaccine development. Here we describe a method which may enable vaccine and adjuvant developers to compare antigen/adjuvant combinations in a harmonized fashion. Three reference antigens: Plasmodium falciparum apical membrane antigen 1 (AMA1), hepatitis B virus surface antigen (HBsAg), and Mycobacterium tuberculosis antigen 85A (Ag85A), were selected as model antigens and were each formulated with three adjuvants: aluminium oxyhydroxide, squalene-in-water emulsion, and a liposome formulation mixed with the purified saponin fraction QS21.Results: The nine antigen/adjuvant formulations were assessed for stability and immunogenicity in mice in order to provide benchmarks against which other formulations could be compared, in order to assist subsequent down selection of adjuvanted vaccines. Furthermore, mouse cellular immune responses were analyzed by measuring IFN-γ and IL-5 production in splenocytes by ELISPOT, and humoral responses were determined by antigen-specific ELISA, where levels of total IgG, IgG1, IgG2b and IgG2c in serum samples were determined.Conclusions: The reference antigens and adjuvants described in this study, which span a spectrum of immune responses, are of potential use as tools to act as points of reference in vaccine development studies. The harmonized methodology described herein may be used as a tool for adjuvant/antigen comparison studies.
- Subjects :
- 0301 basic medicine
Enzyme-Linked Immunospot Assay
HBsAg
Ag85A
medicine.medical_treatment
Protozoan Proteins
0302 clinical medicine
030212 general & internal medicine
Vaccines
Acyltransferases/immunology
Adjuvants, Immunologic/analysis
Animals
Antigens, Bacterial/immunology
Antigens, Protozoan/immunology
Enzyme-Linked Immunosorbent Assay/methods
Enzyme-Linked Immunospot Assay/methods
Hepatitis B Surface Antigens/immunology
Immunization
Immunoglobulin G/blood
Immunoglobulin G/immunology
Interferon-gamma/immunology
Interferon-gamma/metabolism
Interleukin-5/immunology
Interleukin-5/metabolism
Membrane Proteins/immunology
Mice, Inbred C57BL
Protozoan Proteins/immunology
Reproducibility of Results
Spleen/cytology
Spleen/immunology
Spleen/metabolism
Vaccines/analysis
Vaccines/immunology
AMA1
Adjuvants
Aluminium oxyhydroxide
Hepatitis B
Plasmodium falciparum
QS21
Squalene-in-water SWE
Tuberculosis
ELISPOT
Immunogenicity
3. Good health
Vaccination
Adjuvant
Research Article
lcsh:Immunologic diseases. Allergy
Immunology
Antigens, Protozoan
Enzyme-Linked Immunosorbent Assay
Interferon-gamma
03 medical and health sciences
Adjuvants, Immunologic
Antigen
medicine
Apical membrane antigen 1
Antigens, Bacterial
Hepatitis B Surface Antigens
business.industry
Membrane Proteins
030104 developmental biology
Immunoglobulin G
Interleukin-5
business
lcsh:RC581-607
Acyltransferases
Spleen
Subjects
Details
- Language :
- English
- ISSN :
- 14712172
- Volume :
- 19
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- BMC Immunology
- Accession number :
- edsair.doi.dedup.....191a48a61837579b8193ec1700ee03f9