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Detection of Toxoplasma gondii DNA and specific antibodies in high-risk pregnant women
- Source :
- American Journal of Tropical Medicine and Hygiene, American Journal of Tropical Medicine and Hygiene, American Society of Tropical Medicine and Hygiene, 2004, 71 (6), pp.831-5, ResearcherID, Europe PubMed Central
- Publication Year :
- 2005
-
Abstract
- Primary maternal infection with toxoplasmosis during pregnancy is frequently associated with transplacental transmission to the fetus. This study was conducted to test the utility of a polymerase chain reaction (PCR) assay to detect recent infections with Toxoplasma in pregnant women. One hundred forty-eight women with high-risk pregnancies who had abnormal pregnancy outcomes (cases) and 100 with normal pregnancies (controls) were tested for the presence of Toxoplasma DNA in their blood by a nested PCR and specific antibodies to Toxoplasma by an enzyme-linked immunosorbent assay. The IgG results of the cases differed significantly from those of the controls (54% and 12%, respectively; P < 0.02). Four (2.7%) of the cases were IgM positive, but none of the controls were positive. Detection of Toxoplasma DNA in 20 (8.1%) of the IgG-positive cases suggests a recent infection. The risk factors associated with the infection were eating raw meat and contact with soil. The diagnostic serology of recent infection in early pregnancy could be confirmed by a positive Toxoplasma-specific PCR result in blood samples collected in the first half of pregnancy, even in the presence of serologic results difficult to interpret due to the lack of sequential follow-up during pregnancy.
- Subjects :
- Abnormal Pregnancy
Antibodies, Protozoan
Polymerase Chain Reaction
MESH: Pregnancy Complications, Parasitic
Serology
0302 clinical medicine
MESH: Pregnancy
MESH: Risk Factors
Antibody Specificity
Pregnancy
Risk Factors
MESH: Animals
MESH: Immunoglobulin G
0303 health sciences
MESH: Toxoplasma
MESH: Enzyme-Linked Immunosorbent Assay
MESH: Immunoglobulin M
3. Good health
Infectious Diseases
MESH: Toxoplasmosis
Gestation
Female
Toxoplasma
Toxoplasmosis
Adult
Transplacental transmission
030231 tropical medicine
MESH: DNA, Protozoan
Enzyme-Linked Immunosorbent Assay
Biology
03 medical and health sciences
Virology
medicine
MESH: Antibodies, Protozoan
Animals
Humans
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
MESH: Antibody Specificity
MESH: Humans
030306 microbiology
Toxoplasma gondii
MESH: Adult
MESH: Polymerase Chain Reaction
DNA, Protozoan
medicine.disease
biology.organism_classification
Immunoglobulin M
Immunoglobulin G
Pregnancy Complications, Parasitic
Immunology
Parasitology
Nested polymerase chain reaction
MESH: Female
Subjects
Details
- ISSN :
- 00029637
- Volume :
- 71
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- The American journal of tropical medicine and hygiene
- Accession number :
- edsair.doi.dedup.....191832c81306b2a54fa7d3caa85f3f82