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Enhanced expression of adipocyte-type fatty acid binding protein in murine lymphocytes in response to dexamethasone treatment
- Source :
- Molecular and Cellular Biochemistry. 299:99-107
- Publication Year :
- 2006
- Publisher :
- Springer Science and Business Media LLC, 2006.
-
Abstract
- Fatty acids have a great influence on the process of lymphocyte apoptosis which is considered as a modulating factor of immune response in both humans and animals. However the mechanism underlying the function of fatty acids in the process of lymphocyte apoptosis is not fully understood. In this study we show that the appearance of adipocyte-type fatty acid binding protein (A-FABP) is induced upon administration of dexamethasone (DEX) in both in vivo and cultured lymphocytes, and its distinct nuclear localization occurs in close relation to the DEX-induced apoptosis process. In immuunohistochemistry of mouse spleen, A-FABP-immunoreactivity starts to occur 3 h after DEX stimulation, and it massively localizes in the nucleus 8 h after the treatment, while no A-FABP-immunoreactivity is discerned in the lymphocytes of normal as well as 24 h post-injection spleen. In the murine T-cell leukemia CTLL-2 cells, A-FABP-immunoreactivity is also induced in both of the cytoplasm and nucleus when the apoptosis is induced by IL-2 retrieval together with DEX treatment, while in the presence of IL-2 A-FABP-immunoreactivity is confined to the cytoplasm with DEX trreatment. On the other hand, A-FABP-immunoreactivity is not detected by IL-2 retrieval alone. The present findings altogether suggest that A-FABP and its ligands, fatty acids, play an important role in the process of apoptosis and the immune modulation induced by DEX.
- Subjects :
- Male
Lymphocyte
Clinical Biochemistry
Apoptosis
Spleen
Biology
Fatty Acid-Binding Proteins
Dexamethasone
Fatty acid-binding protein
Mice
Immune system
In vivo
Cell Line, Tumor
Adipocytes
medicine
Animals
Lymphocytes
Molecular Biology
DNA Primers
Base Sequence
Cell Biology
General Medicine
Cell biology
Mice, Inbred C57BL
medicine.anatomical_structure
Biochemistry
Cytoplasm
Cell culture
lipids (amino acids, peptides, and proteins)
Subjects
Details
- ISSN :
- 15734919 and 03008177
- Volume :
- 299
- Database :
- OpenAIRE
- Journal :
- Molecular and Cellular Biochemistry
- Accession number :
- edsair.doi.dedup.....18fb99cdeb52a7c16a7ed18067f98627