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A novel screening method detects herpesviral DNA in the idiopathic pulmonary fibrosis lung
- Source :
- University of Helsinki
- Publication Year :
- 2011
- Publisher :
- Informa UK Limited, 2011.
-
Abstract
- Herpesviruses could contribute to the lung epithelial injury that initiates profibrotic responses in idiopathic pulmonary fibrosis (IPF).We identified herpesviral DNA from IPF and control lung tissue using a multiplex PCR-and microarray-based method. Active herpesviral infection was detected by standard methods, and inflammatory cell subtypes were identified with specific antibodies. Patients that underwent lung transplantation were monitored for signs of herpesviral infection.A total of 11/12 IPF samples were positive for Epstein-Barr virus (EBV) and 10/12 for human herpesvirus 6B (HHV-6B) DNA. Control lung samples (n = 10) were negative for EBV DNA, whereas three samples were positive for HHV-6B. EBV-encoded RNA (EBER) was identified in nine IPF samples and localized mainly to lymphocytic aggregates. HHV-6B antigens were detected in mononuclear cells in IPF lung tissue. CD20+ B lymphocytic aggregates that were surrounded by CD3+ T cells were abundant in IPF lungs. CD23+ cells (activated B cells, EBV-transformed lymphoblasts, and dendritic cells) were observed in the aggregates. IPF patients had no signs of increased herpesviral activation after lung transplantation.Inflammatory cells are the main source of herpesviral DNA in the human IPF lung. Diagnostic tools should be actively used to elucidate whether herpesviral infection affects the pathogenesis, progression, and/or exacerbation of IPF.
- Subjects :
- Adult
Male
Epstein-Barr Virus Infections
Herpesvirus 4, Human
Pathology
medicine.medical_specialty
Microarray
Herpesvirus 6, Human
medicine.medical_treatment
Roseolovirus Infections
Peripheral blood mononuclear cell
Virus
03 medical and health sciences
Idiopathic pulmonary fibrosis
0302 clinical medicine
Antigen
medicine
Humans
Lung transplantation
Lung
Aged
030304 developmental biology
CD20
0303 health sciences
biology
General Medicine
Middle Aged
respiratory system
medicine.disease
Idiopathic Pulmonary Fibrosis
respiratory tract diseases
3. Good health
medicine.anatomical_structure
030228 respiratory system
DNA, Viral
Immunology
biology.protein
Female
Multiplex Polymerase Chain Reaction
Lung Transplantation
Subjects
Details
- ISSN :
- 13652060 and 07853890
- Volume :
- 44
- Database :
- OpenAIRE
- Journal :
- Annals of Medicine
- Accession number :
- edsair.doi.dedup.....18c46daea51cb183001200a7013b6a50
- Full Text :
- https://doi.org/10.3109/07853890.2010.532151