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A novel screening method detects herpesviral DNA in the idiopathic pulmonary fibrosis lung

Authors :
Juha Kere
Anne J. Jääskeläinen
Ulla Hodgson
Maija Halme
Irmeli Lautenschlager
Eva Sutinen
Ville Pulkkinen
Maija Lappalainen
Vuokko L. Kinnula
Juho T. Lehto
Heli Piiparinen
Marjukka Myllärniemi
Kaisa Salmenkivi
Source :
University of Helsinki
Publication Year :
2011
Publisher :
Informa UK Limited, 2011.

Abstract

Herpesviruses could contribute to the lung epithelial injury that initiates profibrotic responses in idiopathic pulmonary fibrosis (IPF).We identified herpesviral DNA from IPF and control lung tissue using a multiplex PCR-and microarray-based method. Active herpesviral infection was detected by standard methods, and inflammatory cell subtypes were identified with specific antibodies. Patients that underwent lung transplantation were monitored for signs of herpesviral infection.A total of 11/12 IPF samples were positive for Epstein-Barr virus (EBV) and 10/12 for human herpesvirus 6B (HHV-6B) DNA. Control lung samples (n = 10) were negative for EBV DNA, whereas three samples were positive for HHV-6B. EBV-encoded RNA (EBER) was identified in nine IPF samples and localized mainly to lymphocytic aggregates. HHV-6B antigens were detected in mononuclear cells in IPF lung tissue. CD20+ B lymphocytic aggregates that were surrounded by CD3+ T cells were abundant in IPF lungs. CD23+ cells (activated B cells, EBV-transformed lymphoblasts, and dendritic cells) were observed in the aggregates. IPF patients had no signs of increased herpesviral activation after lung transplantation.Inflammatory cells are the main source of herpesviral DNA in the human IPF lung. Diagnostic tools should be actively used to elucidate whether herpesviral infection affects the pathogenesis, progression, and/or exacerbation of IPF.

Details

ISSN :
13652060 and 07853890
Volume :
44
Database :
OpenAIRE
Journal :
Annals of Medicine
Accession number :
edsair.doi.dedup.....18c46daea51cb183001200a7013b6a50
Full Text :
https://doi.org/10.3109/07853890.2010.532151