Back to Search
Start Over
A fluorescent chemical probe CDy9 selectively stains and enables the isolation of live naïve mouse embryonic stem cells
- Source :
- Biomaterials. 180:12-23
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- Human and mouse embryonic stem cells (ESCs) differ in terms of their pluripotency status, i.e., naive vs. primed. This affects various biological properties and leads to several technical hurdles for future clinical applications, such as difficulties in chimera formation, single-cell passaging, and gene editing. In terms of generating functional human tissues and organs via mammalian interspecies chimerism, a fluorescent chemical probe that specifically labels naive ESCs would help to isolate these cells and monitor their conversion. This study demonstrates that the fluorescent chemical probe compound of designation yellow 9 (CDy9) selectively stains naive, but not primed, mouse ESCs (mESCs). CDy9 entered cells via Slc13a5, a highly expressed membrane transporter in naive mESCs. Fluorescence-based cell sorting based on CDy9 staining successfully separated naive mESCs from primed mESCs. Mice generated using CDy9+ cells isolated during the conversion of mouse epiblast stem cells into naive mESCs exhibited coat color chimerism. Furthermore, CDy9 specifically stained cells in the inner cell mass of mouse embryos. These findings suggest that CDy9 is a useful tool to isolate functional naive mESCs.
- Subjects :
- Boron Compounds
0301 basic medicine
Biophysics
Bioengineering
Biomaterials
Mice
03 medical and health sciences
Chimera (genetics)
0302 clinical medicine
Animals
Inner cell mass
Cells, Cultured
Embryonic Stem Cells
Fluorescent Dyes
Dicarboxylic Acid Transporters
Symporters
Chemistry
Mouse Embryonic Stem Cells
Embryo
Cell sorting
Embryonic stem cell
Cell biology
Staining
030104 developmental biology
Mechanics of Materials
Epiblast
Ceramics and Composites
Stem cell
Heterocyclic Compounds, 3-Ring
Germ Layers
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 01429612
- Volume :
- 180
- Database :
- OpenAIRE
- Journal :
- Biomaterials
- Accession number :
- edsair.doi.dedup.....18c1aeaa896f1cee1fa50578c24b0837