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The Sustainable Release of Vancomycin and Its Degradation Products From Nanostructured Collagen/Hydroxyapatite Composite Layers
- Source :
- Journal of Pharmaceutical Sciences. 105:1288-1294
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- Infections of the musculoskeletal system present a serious problem with regard to the field of orthopedic and trauma medicine. The aim of the experiment described in this study was to develop a resorbable nanostructured composite layer with the controlled elution of antibiotics. The layer is composed of collagen, hydroxyapatite nanoparticles, and vancomycin hydrochloride (10 wt%). The stability of the collagen was enhanced by means of cross-linking. Four cross-linking agents were studied, namely an ethanol solution, a phosphate buffer solution of N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide, genipin, and nordihydroguaiaretic acid. High performance liquid chromatography was used so as to characterize the in vitro release rates of the vancomycin and its crystalline degradation antibiotically inactive products over a 21-day period. The maximum concentration of the released active form of vancomycin (approximately 265 mg/L) exceeded the minimum inhibitory concentration up to an order of 17 times without triggering the burst releasing effect. At the end of the experiment, the minimum inhibitory concentration was exceeded by up to 6 times (approximately 100 mg/L). It was determined that the modification of collagen with hydroxyapatite nanoparticles does not negatively influence the sustainable release of vancomycin. The balance of vancomycin and its degradation products was observed after 14 days of incubation.
- Subjects :
- Hydrochloride
Vancomycin Hydrochloride
Pharmaceutical Science
02 engineering and technology
010402 general chemistry
01 natural sciences
High-performance liquid chromatography
Methylamines
Minimum inhibitory concentration
chemistry.chemical_compound
Drug Delivery Systems
Vancomycin
medicine
Drug Carriers
Chromatography
Chemistry
Elution
021001 nanoscience & nanotechnology
Controlled release
Nanostructures
0104 chemical sciences
Carbodiimides
Durapatite
Delayed-Action Preparations
Genipin
Nanoparticles
Collagen
0210 nano-technology
medicine.drug
Subjects
Details
- ISSN :
- 00223549
- Volume :
- 105
- Database :
- OpenAIRE
- Journal :
- Journal of Pharmaceutical Sciences
- Accession number :
- edsair.doi.dedup.....18b554bd6629d58445a4ba51f99df157