Back to Search
Start Over
Heterologous functional analysis of the Malus xiaojinensis MxIRT1 gene and the His-box motif by expression in yeast
- Source :
- Molecular biology reports. 40(2)
- Publication Year :
- 2012
-
Abstract
- Malus xiaojinensis is an important, iron-efficient rootstock germplasm. Iron uptake is an elaborately controlled process in plant roots, involving specialized transporters. MxIRT1, a Fe(II) transporter gene of M. xiaojinensis, is homologous to other iron transporters at the amino acid level. In the current study, the plasmid pYES2.0-MxIRT1, containing MxIRT1 cDNA, was constructed and transformed into yeast mutants. The results indicated that it could reverse the phenotype of yeast strain DEY1453, an iron uptake mutant. Complementation tests suggested that it might not be a specific transporter, as it was able to restore the phenotypes of other yeast mutant strains, including Mn, Cu and Zn uptake mutants. The functions of the critical histidine residues in the His-box of MxIRT1 were tested by transforming mutant yeast strain DEY1453 with different His residues altered by directed mutagenesis. The His-box of MxIRT1 was found to be necessary for iron transport, with different histidine residues (H(1-4)) playing different roles in the transport.
- Subjects :
- Iron
Mutant
Amino Acid Motifs
Molecular Sequence Data
General Medicine
Saccharomyces cerevisiae
Biology
Yeast
Recombinant Proteins
Complementation
Directed mutagenesis
Plasmid
Biochemistry
Complementary DNA
Malus
Genetics
Mutagenesis, Site-Directed
Amino Acid Sequence
Site-directed mutagenesis
Molecular Biology
Cation Transport Proteins
Histidine
Plant Proteins
Subjects
Details
- ISSN :
- 15734978
- Volume :
- 40
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Molecular biology reports
- Accession number :
- edsair.doi.dedup.....189a758c30490009b1296d6d9b1fa92b