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TRPC1 is regulated by caveolin-1 and is involved in oxidized LDL-induced apoptosis of vascular smooth muscle cells
- Source :
- Journal of Cellular and Molecular Medicine, Journal of Cellular and Molecular Medicine, Wiley Open Access, 2009, 13 (8B), pp.1620-31, Journal of Cellular and Molecular Medicine, 2009, 13 (8B), pp.1620-31
- Publication Year :
- 2009
- Publisher :
- HAL CCSD, 2009.
-
Abstract
- International audience; Oxidized low-density lipoprotein (oxLDL) induced-apoptosis of vascular cells may participate in plaque instability and rupture. We have previously shown that vascular smooth muscle cells (VSMC) stably expressing caveolin-1 were more susceptible to oxLDL-induced apoptosis than VSMC expressing lower level of caveolin-1, and this was correlated with enhanced Ca(2+) entry and pro-apoptotic events. In this study, we aimed to identify the molecular events involved in oxLDL-induced Ca(2+) influx and their regulation by the structural protein caveolin-1. In VSMC, transient receptor potential canonical-1 (TRPC1) silencing by ARN interference prevents the Ca(2+) influx and reduces the toxicity induced by oxLDL. Moreover, caveolin-1 silencing induces concomitant decrease of TRPC1 expression and reduces oxLDL-induced apoptosis of VSMC. OxLDL enhanced the cell surface expression of TRPC1, as shown by biotinylation of cell surface proteins, and induced TRPC1 translocation into caveolar compartment, as assessed by subcellular fractionation. OxLDL-induced TRPC1 translocation was dependent on actin cytoskeleton and associated with a dramatic rise of 7-ketocholesterol (a major oxysterol in oxLDL) into caveolar membranes, whereas the caveolar content of cholesterol was unchanged. Altogether, the reported results show that TRPC1 channels play a role in Ca(2+) influx and Ca(2+) homeostasis deregulation that mediate apoptosis induced by oxLDL. These data also shed new light on the role of caveolin-1 and caveolar compartment as important regulators of TRPC1 trafficking to the plasma membrane and apoptotic processes that play a major role in atherosclerosis.
- Subjects :
- MESH: Lipoproteins, LDL
Vascular smooth muscle
Oxysterol
Caveolin 1
Apoptosis
Biology
MESH: Base Sequence
MESH : RNA, Small Interfering
Muscle, Smooth, Vascular
TRPC1
Transient receptor potential channel
MESH: RNA, Small Interfering
MESH : Cells, Cultured
Humans
MESH: Caveolin 1
RNA, Small Interfering
MESH: TRPC Cation Channels
Cells, Cultured
TRPC Cation Channels
MESH: Humans
Base Sequence
MESH : Gene Expression Regulation
MESH: Apoptosis
MESH : Humans
MESH : TRPC Cation Channels
MESH : Muscle, Smooth, Vascular
Articles
Cell Biology
MESH: Muscle, Smooth, Vascular
Actin cytoskeleton
MESH: Gene Expression Regulation
Cell biology
Lipoproteins, LDL
Gene Expression Regulation
MESH : Caveolin 1
Molecular Medicine
lipids (amino acids, peptides, and proteins)
MESH : Base Sequence
MESH : Lipoproteins, LDL
Homeostasis
MESH : Apoptosis
MESH: Cells, Cultured
Subjects
Details
- Language :
- English
- ISSN :
- 15821838 and 15824934
- Database :
- OpenAIRE
- Journal :
- Journal of Cellular and Molecular Medicine, Journal of Cellular and Molecular Medicine, Wiley Open Access, 2009, 13 (8B), pp.1620-31, Journal of Cellular and Molecular Medicine, 2009, 13 (8B), pp.1620-31
- Accession number :
- edsair.doi.dedup.....1887d0b4dd2f78cb680e86ad97f04301