Back to Search
Start Over
Transfection of exogenous DNA complexed to cationic dendrimer induces alterations of bovine sperm microRNAome
- Source :
- Scopus, Repositório Institucional da UNESP, Universidade Estadual Paulista (UNESP), instacron:UNESP
- Publication Year :
- 2020
-
Abstract
- Made available in DSpace on 2020-12-12T02:14:00Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-10-15 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) MicroRNAs have been hypothesized to be involved in the regulation of male fertility potential. The primary aim of our study was to demonstrate the effects of transfection with dendrimer nanostructure on the parameters of bovine sperm quality and to investigate whether the microRNA profile could be disturbed after cationic dendrimer-mediated exogenous DNA transfection of bovine spermatozoa. The binding of exogenous DNA was significantly increased when dendrimer-based transfection was implemented. However, cationic dendrimer transfection induced detrimental changes in the kinetics and sperm quality parameters, such as membrane integrity, acrosome reaction, and mitochondrial membrane potential, when compared to the control group. Sperm microRNA sequencing revealed 218 known and 106 novel microRNAs in the sperm samples, among which nine were dysregulated after transfection (one was upregulated and eight were downregulated), in comparison to the non-transfected sperm. All the dysregulated microRNAs were related to sperm quality and embryonic development. These results suggest that the transfection process using the dendrimer nanostructure has an impact on the quality and microRNA profile of bovine sperm. Laboratório de Genômica Estrutural Programa de Pós-Graduação em Biotecnologia Centro de Desenvolvimento Tecnológico Universidade Federal de Pelotas Laboratório de Reprodução Animal Programa de Pós-Graduação em Veterinária Universidade Federal de Pelotas Laboratório de Bioinformática e Proteômica Programa de Pós-Graduação em Biotecnologia Centro de Desenvolvimento Tecnológico Universidade Federal de Pelotas Laboratório de Biotecnologia do Câncer Programa de Pós-Graduação em Biotecnologia Centro de Desenvolvimento Tecnológico Universidade Federal de Pelotas Laboratório Genômica e Evolução Molecular Departamento de Genética Instituto de Biociências de Botucatu Universidade Estadual Paulista (UNESP) Unidad de Biología Molecular Institut Pasteur Montevideo Faculdade de Nutrição Universidade Federal de Pelotas Department of Biopharmaceutical Sciences Programs in Human Genetics and Biological Sciences University of California Laboratório Genômica e Evolução Molecular Departamento de Genética Instituto de Biociências de Botucatu Universidade Estadual Paulista (UNESP) Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul: 17/2551-0000953-3 CAPES: AUXPE 2537/2018 FAPESP: FAPERGS-FAPESP #19/2551-0002283-2
- Subjects :
- Male
Dendrimers
animal structures
viruses
Acrosome reaction
Transfection
Non-coding RNAs
Food Animals
Pregnancy
Dendrimer
microRNA
Animals
Small Animals
Gene transfer
MicroRNA sequencing
urogenital system
Equine
Chemistry
Acrosome Reaction
fungi
Embryogenesis
Epigenetic
DNA
Sperm
Spermatozoa
Cell biology
embryonic structures
Animal Science and Zoology
Exogenous DNA
Cattle
Female
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Scopus, Repositório Institucional da UNESP, Universidade Estadual Paulista (UNESP), instacron:UNESP
- Accession number :
- edsair.doi.dedup.....187d762a30e6db1069fb1d5b937da4ee