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Fully automated chemiluminescence vs RIA aldosterone assay in primary aldosteronism work-up
- Source :
- Journal of human hypertension. 31(12)
- Publication Year :
- 2017
-
Abstract
- Aldosterone and renin measurement is a cornerstone for primary aldosteronism (PA) diagnosis, but different thresholds are used according to different assays. A fully automated chemiluminescence (CL) immunoassay for renin and aldosterone was recently proposed, showing good performance for PA screening by aldosterone to renin ratio (ARR). This study aimed to define the accuracy of this assay in the screening and in the most popular confirmatory test of autonomous aldosterone production, the intravenous saline loading test (ivSLT). We compared aldosterone results obtained by CL vs radioimmunoassay (RIA) in hypertensive patients investigated for PA (102 baseline and 85 after ivSLT). An excellent correlation was observed between RIA and CL in the entire population for aldosterone (r=0.922) and ARR (r=0.977). For ARR, Deming regression proved a good accordance between methods and, consistent with the fit model, our previous institutional ARR cut-off of 32 (pg ml−1)/(pg ml−1) corresponded to 20 pg ml−1 mU−1 l−1 in CL assay. However, the correlation was weaker in the low end of aldosterone concentrations (r=0.676 for aldosterone
- Subjects :
- hypertenson
Adult
Male
primary aldosteronism
diagnosis
renin
aldosterone
chemiluminescence
radioimmunoassay
patients
medicine.medical_specialty
Radioimmunoassay
030209 endocrinology & metabolism
030204 cardiovascular system & hematology
law.invention
03 medical and health sciences
chemistry.chemical_compound
Deming regression
0302 clinical medicine
Primary aldosteronism
law
Internal medicine
Renin–angiotensin system
Hyperaldosteronism
Internal Medicine
medicine
Humans
Aldosterone
Chemiluminescence
Aged
Aldosterone-to-renin ratio
medicine.diagnostic_test
business.industry
Middle Aged
medicine.disease
Endocrinology
chemistry
Immunoassay
Luminescent Measurements
Female
business
Subjects
Details
- ISSN :
- 14765527
- Volume :
- 31
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- Journal of human hypertension
- Accession number :
- edsair.doi.dedup.....187586eaa654772f5f9b66200f699555