Back to Search
Start Over
An integrated expression atlas of miRNAs and their promoters in human and mouse
- Source :
- Nature biotechnology, vol 35, iss 9, Nature biotechnology 35(9), 872-878 (2017). doi:10.1038/nbt.3947, Nature Biotechnology, Nature Biotechnology, Nature Publishing Group, 2017, 35 (9), pp.872-878. ⟨10.1038/nbt.3947⟩, de Rie, D 2017, ' An integrated expression atlas of miRNAs and their promoters in human and mouse ', Nature Biotechnology, vol. 35, no. 9, pp. 872-878 . https://doi.org/10.1038/nbt.3947
- Publication Year :
- 2017
- Publisher :
- eScholarship, University of California, 2017.
-
Abstract
- International audience; MicroRNAs (miRNAs) are short non-coding RNAs with key roles in cellular regulation. As part of the fifth edition of the Functional Annotation of Mammalian Genome (FANTOM5) project, we created an integrated expression atlas of miRNAs and their promoters by deep-sequencing 492 short RNA (sRNA) libraries, with matching Cap Analysis Gene Expression (CAGE) data, from 396 human and 47 mouse RNA samples. Promoters were identified for 1,357 human and 804 mouse miRNAs and showed strong sequence conservation between species. We also found that primary and mature miRNA expression levels were correlated, allowing us to use the primary miRNA measurements as a proxy for mature miRNA levels in a total of 1,829 human and 1,029 mouse CAGE libraries. We thus provide a broad atlas of miRNA expression and promoters in primary mammalian cells, establishing a foundation for detailed analysis of miRNA expression patterns and transcriptional control regions.
- Subjects :
- 0301 basic medicine
FANTOM Consortium
Cells
Biomedical Engineering
Bioengineering
Biology
Applied Microbiology and Biotechnology
Genome
Article
Promoter Regions
03 medical and health sciences
Mice
Genetic
Transcription (biology)
metabolism [MicroRNAs]
[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN]
microRNA
Gene expression
Transcriptional regulation
Gene silencing
Animals
Humans
genetics [MicroRNAs]
Promoter Regions, Genetic
Cells, Cultured
Gene Library
Genetics
Cultured
Gene Expression Profiling
RNA
High-Throughput Nucleotide Sequencing
Molecular Sequence Annotation
Gene expression profiling
MicroRNAs
030104 developmental biology
Good Health and Well Being
genetics [Promoter Regions, Genetic]
ddc:660
Molecular Medicine
Generic health relevance
Biotechnology
methods [Gene Expression Profiling]
Subjects
Details
- ISSN :
- 10870156
- Database :
- OpenAIRE
- Journal :
- Nature biotechnology, vol 35, iss 9, Nature biotechnology 35(9), 872-878 (2017). doi:10.1038/nbt.3947, Nature Biotechnology, Nature Biotechnology, Nature Publishing Group, 2017, 35 (9), pp.872-878. ⟨10.1038/nbt.3947⟩, de Rie, D 2017, ' An integrated expression atlas of miRNAs and their promoters in human and mouse ', Nature Biotechnology, vol. 35, no. 9, pp. 872-878 . https://doi.org/10.1038/nbt.3947
- Accession number :
- edsair.doi.dedup.....1873e9fffa1b7e1d9b1648f6e84c5cad