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Multiplex Polymerase Chain Reaction for Identification and Differentiation of Streptococcus equi subsp. zooepidemicus and Streptococcus equi subsp. equi
- Source :
- Journal of Veterinary Medicine Series B. 51:455-458
- Publication Year :
- 2004
- Publisher :
- Wiley, 2004.
-
Abstract
- Summary The closely related streptococcal species Streptococcus equi subsp. zooepidemicus and S. equi subsp. equi were identified by polymerase chain reaction using oligonucleotide primers designed according to species-specific parts of the superoxide dismutase A encoding gene sodA. A further differentiation of both subspecies could be performed by amplification of the genes seeH and seeI encoding the exotoxins SeeH and SeeI, respectively, which could be detected for S. equi subsp. equi but not for S. equi subsp. zooepidemicus. A further simplification of the identification and differentiation of both subspecies was conducted by sodA–seeI multiplex polymerase chain reaction.
- Subjects :
- Streptococcus equi
animal diseases
Biology
Subspecies
Polymerase Chain Reaction
Microbiology
law.invention
Predictive Value of Tests
law
Germany
RNA, Ribosomal, 16S
Streptococcal Infections
parasitic diseases
Multiplex polymerase chain reaction
Animals
Horses
Gene
Phylogeny
Polymerase chain reaction
DNA Primers
General Medicine
bacterial infections and mycoses
Virology
respiratory tract diseases
Oligonucleotide primers
Streptococcus equi subsp. zooepidemicus
Genes, Bacterial
bacteria
Horse Diseases
Streptococcus equi subsp equi
Subjects
Details
- ISSN :
- 14390450 and 09311793
- Volume :
- 51
- Database :
- OpenAIRE
- Journal :
- Journal of Veterinary Medicine Series B
- Accession number :
- edsair.doi.dedup.....186eaac66bc1d807f840fdb88ecd8cb1