Back to Search Start Over

Critical Impact of Human Amniotic Membrane Tension on Mitochondrial Function and Cell Viability In Vitro

Authors :
Katy Schmidt
Susanne Wolbank
Laura Poženel
Andrea Lindenmair
Asmita Banerjee
Andrey V. Kozlov
Adelheid Weidinger
Johannes Grillari
Source :
Cells
Publication Year :
2019
Publisher :
MDPI AG, 2019.

Abstract

Amniotic cells show exciting stem cell features, which has led to the idea of using living cells of human amniotic membranes (hAMs) in toto for clinical applications. However, under common cell culture conditions, viability of amniotic cells decreases rapidly, whereby reasons for this decrease are unknown so far. Recently, it has been suggested that loss of tissue tension in vivo leads to apoptosis. Therefore, the aim of this study was to investigate the effect of tissue distention on the viability of amniotic cells in vitro. Thereby, particular focus was put on vital mitochondria-linked parameters, such as respiration and ATP synthesis. Biopsies of hAMs were incubated for 7–21 days either non-distended or distended. We observed increased B-cell lymphoma 2-associated X protein (BAX)/B-cell lymphoma (BCL)-2 ratios in non-distended hAMs at day seven, followed by increased caspase 3 expression at day 14, and, consequently, loss of viability at day 21. In contrast, under distention, caspase 3 expression increased only slightly, and mitochondrial function and cellular viability were largely maintained. Our data suggest that a mechano-sensing pathway may control viability of hAM cells by triggering mitochondria-mediated apoptosis upon loss of tension in vitro. Further studies are required to elucidate the underlying molecular mechanisms between tissue distention and viability of hAM cells.

Details

ISSN :
20734409
Volume :
8
Database :
OpenAIRE
Journal :
Cells
Accession number :
edsair.doi.dedup.....186b448925d3b916713d768d46a5f350
Full Text :
https://doi.org/10.3390/cells8121641