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Production and roles of IAA and ABA during development of superior and inferior rice grains
- Source :
- Functional plant biology : FPB. 47(8)
- Publication Year :
- 2019
-
Abstract
- Current understanding of the role of plant hormones during cereal grain filling is confounded by contradictory reports on hormone production that is based on poor methodology. We report here on the accurate measurement of indole-3-acetic acid (IAA) and abscisic acid (ABA) by combined liquid chromatography-tandem mass spectrometry in multiple reaction-monitoring mode with heavy isotope labelled internal standards. ABA and IAA contents of superior versus inferior rice grains (ABA maxima 159 ng g–1 FW and 109 ng g–1 FW, IAA maxima 2 µg g–1 FW and 1.7 µg g–1 FW respectively) correlated with the expression of biosynthetic genes and with grain fill. Results confirm that grain ABA is produced primarily by OsNCED2(5), but suggest that ABA import and metabolism also play important roles in ABA regulation. The IAA content of grains is primarily influenced by OsYUC9 and OsYUC11. However, the distinct expression profile of OsYUC12 suggests a specific role for IAA produced by this enzyme. Co-expression of OsYUC12 with OsIAA29 indicates their involvement in a common signalling pathway. Co-expression and cis-element analysis identified several aleurone-specific transcriptional regulators as well as glutelin as strong candidates for detailed investigation for direct regulation by the auxin-signalling pathway.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Plant Science
Biology
01 natural sciences
03 medical and health sciences
chemistry.chemical_compound
Plant Growth Regulators
Glutelin
Abscisic acid
chemistry.chemical_classification
Indoleacetic Acids
food and beverages
Oryza
Metabolism
Heavy isotope
Cereal grain
030104 developmental biology
Enzyme
Biochemistry
chemistry
biology.protein
Agronomy and Crop Science
010606 plant biology & botany
Biosynthetic genes
Abscisic Acid
Subjects
Details
- ISSN :
- 14454416
- Volume :
- 47
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Functional plant biology : FPB
- Accession number :
- edsair.doi.dedup.....1867e20100ef0b2f31c9e3c52d3a67ef