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The nucleoside diphosphate kinase NDK-1/NME1 promotes phagocytosis in concert with DYN-1/Dynamin
- Source :
- FASEB Journal, FASEB Journal, Federation of American Society of Experimental Biology, 2019, 33 (10), pp.11606-11614. ⟨10.1096/fj.201900220R⟩, The FASEB Journal
- Publication Year :
- 2019
- Publisher :
- HAL CCSD, 2019.
-
Abstract
- Phagocytosis of various targets, such as apoptotic cells or opsonized pathogens, by macrophages is coordinated by a complex signaling network initiated by distinct phagocytic receptors. Despite the different initial signaling pathways, each pathway ends up regulating the actin cytoskeletal network, phagosome formation and closure, and phagosome maturation leading to degradation of the engulfed particle. Herein, we describe a new phagocytic function for the nucleoside diphosphate kinase 1 (NDK-1), the nematode counterpart of the first identified metastasis inhibitor NM23-H1 (nonmetastatic clone number 23) nonmetastatic clone number 23 or nonmetastatic isoform 1 (NME1). We reveal by coimmunoprecipitation, Duolink proximity ligation assay, and mass spectrometry that NDK-1/NME1 works in a complex with DYN-1/Dynamin (Caenorhabditis elegans/human homolog proteins), which is essential for engulfment and phagosome maturation. Time-lapse microscopy shows that NDK-1 is expressed on phagosomal surfaces during cell corpse clearance in the same time window as DYN-1. Silencing of NM23-M1 in mouse bone marrow–derived macrophages resulted in decreased phagocytosis of apoptotic thymocytes. In human macrophages, NM23-H1 and Dynamin are corecruited at sites of phagosome formation in F-actin–rich cups. In addition, NM23-H1 was required for efficient phagocytosis. Together, our data demonstrate that NDK-1/NME1 is an evolutionarily conserved element of successful phagocytosis.—Farkas, Z., Petric, M., Liu, X., Herit, F., Rajnavölgyi, É., Szondy, Z., Budai, Z., Orbán, T. I., Sándor, S., Mehta, A., Bajtay, Z., Kovács, T., Jung, S. Y., Afaq Shakir, M., Qin, J., Zhou, Z., Niedergang, F., Boissan, M., Takács-Vellai, K. The nucleoside diphosphate kinase NDK-1/NME1 promotes phagocytosis in concert with DYN-1/dynamin.
- Subjects :
- 0301 basic medicine
Dynamins
Phagocytosis
metastasis inhibitor
apoptotic clearance
Apoptosis
[SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC]
Biochemistry
03 medical and health sciences
Mice
0302 clinical medicine
Phagosomes
Phagosome maturation
Genetics
Animals
Humans
Receptor
Caenorhabditis elegans
Caenorhabditis elegans Proteins
Molecular Biology
Opsonin
Cells, Cultured
ComputingMilieux_MISCELLANEOUS
Dynamin
Chemistry
Research
Macrophages
phagosome maturation
NM23 Nucleoside Diphosphate Kinases
Nucleoside-diphosphate kinase
Actins
Cell biology
Mice, Inbred C57BL
Signaling network
030104 developmental biology
Leukocytes, Mononuclear
phagosome formation
030217 neurology & neurosurgery
Biotechnology
actin cup
Signal Transduction
Subjects
Details
- Language :
- English
- ISSN :
- 08926638 and 15306860
- Database :
- OpenAIRE
- Journal :
- FASEB Journal, FASEB Journal, Federation of American Society of Experimental Biology, 2019, 33 (10), pp.11606-11614. ⟨10.1096/fj.201900220R⟩, The FASEB Journal
- Accession number :
- edsair.doi.dedup.....1861d8273bdac2083b3787f2c78aac8c