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Serr spectroelectrochemistry as a guide for rational design of dyp-based bioelectronics devices
- Source :
- Repositório Científico de Acesso Aberto de Portugal, Repositório Científico de Acesso Aberto de Portugal (RCAAP), instacron:RCAAP, International Journal of Molecular Sciences, 22(15):7998. MDPI AG, International Journal of Molecular Sciences, Volume 22, Issue 15, International Journal of Molecular Sciences, Vol 22, Iss 7998, p 7998 (2021), Int J Mol Sci.
- Publication Year :
- 2021
-
Abstract
- Funding Information: Acknowledgments: We acknowledge the support from Project LISBOA-01-0145-FEDER-007660 (Microbiologia Molecular, Estrutural e Celular) funded by FEDER funds through COMPETE 2020-Programa Operacional Competitividade e Internacionalização (POCI), from FCT—Fundação para a Ciência e a Tecnologia (PTDC/BIA-BFS/31026/2017 and 2020.05017.BD) and from the European Union’s Horizon 2020 Research and Innovation Program, through TIMB3 and B-LigZymes projects (grant agreements numbers are 810856 and 824017, respectively). Molecular graphics and analyses were performed with UCSF ChimeraX, developed by the Resource for Biocomputing, Visualization, and Informatics at the University of California, San Francisco, with support from National Institutes of Health R01-GM129325 and the Office of Cyber Infrastructure and Computational Biology, National Institute of Allergy and Infectious Diseases. Funding Information: This research was funded by FCT?Funda??o para a Ci?ncia e a Tecnologia (grant number: PTDC/BIA-BFS/31026/2017). The APC was funded by the TIMB3 project, European Union?s Horizon 2020 Research and Innovation Program (grant agreement No.: 810856). Funding Information: Funding: This research was funded by FCT—Fundação para a Ciência e a Tecnologia (grant number: PTDC/BIA-BFS/31026/2017). The APC was funded by the TIMB3 project, European Union’s Horizon 2020 Research and Innovation Program (grant agreement No.: 810856). Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. Immobilised dye-decolorizing peroxidases (DyPs) are promising biocatalysts for the development of biotechnological devices such as biosensors for the detection of H2O2 . To this end, these enzymes have to preserve native, solution properties upon immobilisation on the electrode surface. In this work, DyPs from Cellulomonas bogoriensis (CboDyP), Streptomyces coelicolor (ScoDyP) and Thermobifida fusca (TfuDyP) are immobilised on biocompatible silver electrodes functionalized with alkanethiols. Their structural, redox and catalytic properties upon immobilisation are evaluated by surface-enhanced resonance Raman (SERR) spectroelectrochemistry and cyclic voltammetry. Among the studied electrode/DyP constructs, only CboDyP shows preserved native structure upon attachment to the electrode. However, a comparison of the redox potentials of the enzyme in solution and immobilised states reveals a large discrepancy, and the enzyme shows no electrocatalytic activity in the presence of H2O2 . While some immobilised DyPs outperform existing peroxidase-based biosensors, others fail to fulfil the essential requirements that guarantee their applicability in the im-mobilised state. The capacity of SERR spectroelectrochemistry for fast screening of the performance of immobilised heme enzymes places it in the front-line of experimental approaches that can advance the search for promising DyP candidates. publishersversion published
- Subjects :
- Immobilised enzymes
QH301-705.5
Biosensing Techniques
02 engineering and technology
010402 general chemistry
01 natural sciences
Redox
Article
Catalysis
Inorganic Chemistry
Bacterial Proteins
Biology (General)
Physical and Theoretical Chemistry
Electrodes
QD1-999
Molecular Biology
Spectroscopy
Peroxidase
Bioelectronics
3rd generation biosensors
Bacteria
biology
Chemistry
Streptomyces coelicolor
Organic Chemistry
Rational design
3 generation biosensors
General Medicine
Enzymes, Immobilized
021001 nanoscience & nanotechnology
biology.organism_classification
Combinatorial chemistry
0104 chemical sciences
Computer Science Applications
SERR spectroelectrochemistry
Cellulomonas bogoriensis
DyP
biology.protein
Cyclic voltammetry
0210 nano-technology
Biosensor
Subjects
Details
- Language :
- English
- ISSN :
- 16616596
- Database :
- OpenAIRE
- Journal :
- Repositório Científico de Acesso Aberto de Portugal, Repositório Científico de Acesso Aberto de Portugal (RCAAP), instacron:RCAAP, International Journal of Molecular Sciences, 22(15):7998. MDPI AG, International Journal of Molecular Sciences, Volume 22, Issue 15, International Journal of Molecular Sciences, Vol 22, Iss 7998, p 7998 (2021), Int J Mol Sci.
- Accession number :
- edsair.doi.dedup.....185aa4a93925ab9c283bbab760ee65d3