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DNA methylation and histone post-translational modification stability in post-mortem brain tissue
- Source :
- Clinical Epigenetics, Vol 11, Iss 1, Pp 1-23 (2019), Clinical Epigenetics
- Publication Year :
- 2019
- Publisher :
- BMC, 2019.
-
Abstract
- Background Epigenetic (including DNA and histone) modifications occur in a variety of neurological disorders. If epigenetic features of brain autopsy material are to be studied, it is critical to understand the post-mortem stability of the modifications. Methods Pig and mouse brain tissue were formalin-fixed and paraffin-embedded, or frozen after post-mortem delays of 0, 24, 48, and 72 h. Epigenetic modifications frequently reported in the literature were studied by DNA agarose gel electrophoresis, DNA methylation enzyme-linked immunosorbent assays, Western blotting, and immunohistochemistry. We constructed a tissue microarray of human neocortex samples with devitalization or death to fixation times ranging from
- Subjects :
- Male
0301 basic medicine
Swine
lcsh:Medicine
Histone methylation
Epigenesis, Genetic
Histones
Mice
chemistry.chemical_compound
0302 clinical medicine
Pig brain
Genetics (clinical)
DNA methylation
Tissue microarray
biology
Brain
Acetylation
3. Good health
Histone
Histone acetylation
030220 oncology & carcinogenesis
Cortex
Epigenetics
Female
Autopsy
Post-mortem delay
lcsh:QH426-470
Human brain
03 medical and health sciences
Genetics
Animals
Humans
Molecular Biology
Mouse brain
Research
lcsh:R
Molecular biology
lcsh:Genetics
030104 developmental biology
chemistry
Tissue Array Analysis
biology.protein
H3K4me3
Protein Processing, Post-Translational
DNA
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 18687083 and 18687075
- Volume :
- 11
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Clinical Epigenetics
- Accession number :
- edsair.doi.dedup.....182891f1470a7f9b06f28aa14ad4d373