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Selective deposition of native cell membranes on biocompatible micropatterns
- Source :
- Journal of the American Chemical Society. 126(10)
- Publication Year :
- 2004
-
Abstract
- We establish two methods to deposit native biomembranes (human erythrocyte membranes and sarcoplasmic reticulum membranes) selectively onto biocompatible microtemplates. The first method utilizes UV photolithography to micropattern the regenerated cellulose, while the second uses the “stamping” of protein barriers onto homogeneous cellulose supports. The relatively simple methods established here allow for the position selective spreading of three-dimensional native cells into two-dimensional films, retaining the orientation and lateral density of transmembrane proteins in their native state.
- Subjects :
- Cell
Biocompatible Materials
Biochemistry
Catalysis
Fluorescence
chemistry.chemical_compound
Colloid and Surface Chemistry
medicine
Native state
Humans
Cellulose
Chromatography
Chemistry
Endoplasmic reticulum
Erythrocyte Membrane
Regenerated cellulose
Serum Albumin, Bovine
General Chemistry
Biocompatible material
Transmembrane protein
Sarcoplasmic Reticulum
medicine.anatomical_structure
Membrane
Biophysics
Glass
Fluorescein-5-isothiocyanate
Subjects
Details
- ISSN :
- 00027863
- Volume :
- 126
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Journal of the American Chemical Society
- Accession number :
- edsair.doi.dedup.....18200048dd3cc4cd27386c5a390e57ca