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Alzheimer's amyloid-β peptide disturbs P2X7 receptor-mediated circadian oscillations of intracellular calcium

Alzheimer's amyloid-β peptide disturbs P2X7 receptor-mediated circadian oscillations of intracellular calcium

Authors :
Amandine Grimm
Anna Wilkaniec
Anne Eckert
Joanna B. Strosznajder
Karen Schmitt
Source :
Folia Neuropathologica, Vol 54, Iss 4, Pp 360-368 (2016)
Publication Year :
2017

Abstract

Recent data indicate that Alzheimer's disease (AD) is associated with disturbances of the circadian rhythm in patients. We examined the effect of amyloid-β (Aβ) peptide, the main component of the senile plaques playing a critical role in the deregulation of calcium (Casup2+/sup) homeostasis in AD, on the circadian oscillation of cytosolic calcium (Casup2+/sup) levelsiiin vitro/i/i. The experiments we carried out in human primary skin fibroblasts. This cell line was previously shown to exhibit circadian rhythms of clock genes. Moreover, the basic clock properties of these peripheral cells closely mimic those measured physiologically and behaviorally in human and do not change during aging. In this study we showed that i) cytosolic Casup2+/suposcillations depend on the activation of purinergic P2X7 receptors; and ii) these oscillations are abolished in the presence of Aβ. In total, our new findings may help to deepen our understanding of the molecular mechanisms involved in AD-related circadian alterations.

Details

ISSN :
1509572X
Volume :
54
Issue :
4
Database :
OpenAIRE
Journal :
Folia neuropathologica
Accession number :
edsair.doi.dedup.....1805c265657d504b56406d88223c24ca