Back to Search
Start Over
Reduced changes in protein compared to mRNA levels across non-proliferating tissues
- Source :
- BMC Genomics, Vol 18, Iss 1, Pp 1-14 (2017), BMC Genomics
- Publication Year :
- 2017
- Publisher :
- BMC, 2017.
-
Abstract
- Background The quantitative relations between RNA and protein are fundamental to biology and are still not fully understood. Across taxa, it was demonstrated that the protein-to-mRNA ratio in steady state varies in a direction that lessens the change in protein levels as a result of changes in the transcript abundance. Evidence for this behavior in tissues is sparse. We tested this phenomenon in new data that we produced for the mouse auditory system, and in previously published tissue datasets. A joint analysis of the transcriptome and proteome was performed across four datasets: inner-ear mouse tissues, mouse organ tissues, lymphoblastoid primate samples and human cancer cell lines. Results We show that the protein levels are more conserved than the mRNA levels in all datasets, and that changes in transcription are associated with translational changes that exert opposite effects on the final protein level, in all tissues except cancer. Finally, we observe that some functions are enriched in the inner ear on the mRNA level but not in protein. Conclusions We suggest that partial buffering between transcription and translation ensures that proteins can be made rapidly in response to a stimulus. Accounting for the buffering can improve the prediction of protein levels from mRNA levels. Electronic supplementary material The online version of this article (doi:10.1186/s12864-017-3683-9) contains supplementary material, which is available to authorized users.
- Subjects :
- 0301 basic medicine
Primates
Translation
Proteome
lcsh:QH426-470
lcsh:Biotechnology
RNA-Seq
Biology
Proteomics
Transcriptome
03 medical and health sciences
Mice
0302 clinical medicine
Transcription (biology)
Neoplasms
lcsh:TP248.13-248.65
Inner ear
Genetics
Animals
Humans
RNA, Messenger
Cell Proliferation
Mass spectrometry
RNA
Cell biology
Cochlea
Gene Expression Regulation, Neoplastic
lcsh:Genetics
030104 developmental biology
Organ Specificity
Ear, Inner
sense organs
DNA microarray
RNA-seq
030217 neurology & neurosurgery
Biotechnology
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 14712164
- Volume :
- 18
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- BMC Genomics
- Accession number :
- edsair.doi.dedup.....1805b0773cc68653f1d51a3300dc423d