Back to Search
Start Over
Distinct processing of the pre-B cell receptor and the B cell receptor
- Source :
- Molecular Immunology. 54:115-121
- Publication Year :
- 2013
- Publisher :
- Elsevier BV, 2013.
-
Abstract
- It has been recently demonstrated that while oligosaccharide moieties of μ heavy chains in the B-cell receptor (BCR) are of the complex type as expected, those of the pre-BCR on the surface of pre-B cells contain oligosaccharide moieties of the high-mannose type only. This is unique, because high-mannose glycans are generally restricted to the endoplasmic reticulum and not presented on the surface of mammalian cells. In the present study, we examined the processing of the unusually glycosylated μ heavy chains in pre-B cells. We demonstrate that the pre-BCR reaches the cell surface by a non-conventional brefeldin A-sensitive monensin-insensitive transport pathway. Although pre-BCR complexes consist of μ heavy chains with high-mannose oligosaccharide moieties, they are stably expressed in the plasma membrane and demonstrate turnover rates similar to those of the BCR. Thus, rapid internalization cannot account for their low surface expression, as previously postulated. Rather, we demonstrate that the low pre-BCR abundance in the plasma membrane results, at least in part, from insufficient production of surrogate light chains, which appears to be a limiting factor in pre-BCR expression.
- Subjects :
- Glycan
Immunoglobulin Light Chains, Surrogate
media_common.quotation_subject
Immunology
Cell
B-cell receptor
Receptors, Antigen, B-Cell
Cell Line
Mice
hemic and lymphatic diseases
medicine
Animals
Monensin
Internalization
Receptor
Molecular Biology
media_common
chemistry.chemical_classification
B-Lymphocytes
Brefeldin A
biology
Precursor Cells, B-Lymphoid
Endoplasmic reticulum
Cell Membrane
breakpoint cluster region
Oligosaccharide
Protein Transport
medicine.anatomical_structure
chemistry
Biochemistry
Pre-B Cell Receptors
biology.protein
Biophysics
Subjects
Details
- ISSN :
- 01615890
- Volume :
- 54
- Database :
- OpenAIRE
- Journal :
- Molecular Immunology
- Accession number :
- edsair.doi.dedup.....17faffd9ccf775f240d8fb62fba8c01a