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Study on the molecular mechanism of Guizhi Jia Shaoyao decoction for the treatment of knee osteoarthritis by utilizing network pharmacology and molecular docking technology
- Source :
- Allergologia et Immunopathologia. 49:16-30
- Publication Year :
- 2021
- Publisher :
- Codon Publications, 2021.
-
Abstract
- Background and objective: Guizhi Jia Shaoyao decoction (GSD) is widely used in the clinical treatment of knee osteoarthritis (KOA). However, the underlying molecular mechanisms remain unclear. The aim of this study was to explore functional mechanisms of GSD in treating KOA by utilizing network pharmacology-based approaches. Methods: Candidate components and targets of GSD were retrieved from the Traditional Chinese Medicine Systems Pharmacology database. NCBI, Genecards, Drugbank, and Therapeutic Target Database (TTD) were used to establish a target database for KOA. Then, an interactive network diagram of “drugs–active components–targets” was plotted with Cytoscape open source bioinformatics software. A protein–protein interaction network was constructed and related protein interaction relationships were analyzed based on the STRING database. Gene ontology analysis and Kyoto Encyclopedia of Genes and Genomes pathway-enrichment analysis were conducted based on intersected targets. Molecular docking provided an assessment tool for verifying binding of components and targets. It was performed by AutoDock molecular modeling simulation software. Results: In all, 103 active components were successfully identified, and corresponding 133 targets were searched for treating KOA. Functional enrichment analysis suggested that GSD exerts its pharmacological effect in treating KOA by regulating multiple pathways, such as PI3K-Akt, tumor necrosis factor, Toll-like receptor (TLR), and nuclear factor kappa B signaling pathways. Molecular docking analysis depicted that representative components bound firmly to key targets. Conclusion: This study revealed the synergistic effects of multiple components, targets, and pathways of GSD for treating KOA. This would enhance the understanding of potential molecular mechanisms of GSD for treating KOA and lay a foundation for further experimental research.
- Subjects :
- Pulmonary and Respiratory Medicine
business.industry
Immunology
General Medicine
Osteoarthritis
Computational biology
Network Pharmacology
Osteoarthritis, Knee
AutoDock
medicine.disease
GeneCards
Molecular Docking Simulation
Interaction network
Network pharmacology
medicine
Humans
Immunology and Allergy
KEGG
business
DrugBank
Drugs, Chinese Herbal
Signal Transduction
Systems pharmacology
Subjects
Details
- ISSN :
- 03010546
- Volume :
- 49
- Database :
- OpenAIRE
- Journal :
- Allergologia et Immunopathologia
- Accession number :
- edsair.doi.dedup.....17f83cb6135499b940689aa24fe46925