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Excretory/secretory antigens of Strongyloides venezuelensis applied to IgG detection in human strongyloidosis

Authors :
José Eduardo Neto de Sousa
Edson Fernando Goulart de Carvalho
Renata Araújo Cunha
Julia Maria Costa-Cruz
Source :
Parasitology international. 66(5)
Publication Year :
2017

Abstract

Strongyloidosis is a neglected disease that affects millions of people around the world. The cases that particularly deserve attention are those related to hyperinfection, mainly in immunocompromised patients. In this sense, there is a need to improve the serological diagnosis of this helminth. The objective of this study was therefore to produce and characterize excretory/secretory (E/S) antigens of Strongyloides venezuelensis infective larvae (L3) for use as a heterologous antigen in the diagnosis of human strongyloidosis and other parasitic infection groups. Soluble antigenic preparations were produced as total saline extract (SE), E/S in Roswell Park Memorial Institute 1640 (RPMI) and E/S in phosphate buffered saline (PBS). The three antigenic preparations showed similar protein bands. An ELISA showed that the E/S antigens were profitable, easy to use, and more sensitive and specific than SE, eliminating cross-reactivity with other parasites in serum samples. The detection of anti-Strongyloides stercoralis in the sera of patients with strongyloidosis and those with immunosuppressive conditions using S. venezuelensis L3 larvae E/S antigens was satisfactory. RPMI and PBS E/S antigens were also superior in terms of specificity than SE.

Details

ISSN :
18730329
Volume :
66
Issue :
5
Database :
OpenAIRE
Journal :
Parasitology international
Accession number :
edsair.doi.dedup.....17e4fd3dd50722eba229b70b53f738a2