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Excretory/secretory antigens of Strongyloides venezuelensis applied to IgG detection in human strongyloidosis
- Source :
- Parasitology international. 66(5)
- Publication Year :
- 2017
-
Abstract
- Strongyloidosis is a neglected disease that affects millions of people around the world. The cases that particularly deserve attention are those related to hyperinfection, mainly in immunocompromised patients. In this sense, there is a need to improve the serological diagnosis of this helminth. The objective of this study was therefore to produce and characterize excretory/secretory (E/S) antigens of Strongyloides venezuelensis infective larvae (L3) for use as a heterologous antigen in the diagnosis of human strongyloidosis and other parasitic infection groups. Soluble antigenic preparations were produced as total saline extract (SE), E/S in Roswell Park Memorial Institute 1640 (RPMI) and E/S in phosphate buffered saline (PBS). The three antigenic preparations showed similar protein bands. An ELISA showed that the E/S antigens were profitable, easy to use, and more sensitive and specific than SE, eliminating cross-reactivity with other parasites in serum samples. The detection of anti-Strongyloides stercoralis in the sera of patients with strongyloidosis and those with immunosuppressive conditions using S. venezuelensis L3 larvae E/S antigens was satisfactory. RPMI and PBS E/S antigens were also superior in terms of specificity than SE.
- Subjects :
- 0301 basic medicine
Antibodies, Helminth
Heterologous
Enzyme-Linked Immunosorbent Assay
Cross Reactions
Immunologic Tests
Sensitivity and Specificity
Immunoglobulin G
Serology
Strongyloides stercoralis
Microbiology
03 medical and health sciences
Immunocompromised Host
Antigen
medicine
Helminths
Animals
Humans
biology
medicine.disease
biology.organism_classification
030104 developmental biology
Infectious Diseases
Strongyloidiasis
Excretory system
Antigens, Helminth
biology.protein
Parasitology
Subjects
Details
- ISSN :
- 18730329
- Volume :
- 66
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Parasitology international
- Accession number :
- edsair.doi.dedup.....17e4fd3dd50722eba229b70b53f738a2