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The structural proteome of Pseudomonas aeruginosa bacteriophage ϕKMV

The structural proteome of Pseudomonas aeruginosa bacteriophage ϕKMV

Authors :
Johan Robben
Yves Briers
Jean-Paul Noben
Bart Roucourt
Vadim V. Mesyanzhinov
Rob Lavigne
Victor N. Krylov
Debora Dumont
Kirsten Hertveldt
Pieter-Jan Ceyssens
Guido Volckaert
Source :
Microbiology. 152:529-534
Publication Year :
2006
Publisher :
Microbiology Society, 2006.

Abstract

The structural proteome of ϕKMV, a lytic bacteriophage infecting Pseudomonas aeruginosa, was analysed using two approaches. In one approach, structural proteins of the phage were fractionated by SDS-PAGE for identification by liquid chromatography-mass spectrometry (LC-MS). In a second approach, a whole-phage shotgun analysis (WSA) was applied. WSA uses trypsin digestion of whole phage particles, followed by reversed-phase HPLC and gas-phase fractionation of the complex peptide mixture prior to MS. The results yield a comprehensive view of structure-related proteins in ϕKMV and suggest subtle structural differences from phage T7.

Details

ISSN :
14652080 and 13500872
Volume :
152
Database :
OpenAIRE
Journal :
Microbiology
Accession number :
edsair.doi.dedup.....17da4623d50e599e1ca34759a66066ec
Full Text :
https://doi.org/10.1099/mic.0.28431-0