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The structural proteome of Pseudomonas aeruginosa bacteriophage ϕKMV
The structural proteome of Pseudomonas aeruginosa bacteriophage ϕKMV
- Source :
- Microbiology. 152:529-534
- Publication Year :
- 2006
- Publisher :
- Microbiology Society, 2006.
-
Abstract
- The structural proteome of ϕKMV, a lytic bacteriophage infecting Pseudomonas aeruginosa, was analysed using two approaches. In one approach, structural proteins of the phage were fractionated by SDS-PAGE for identification by liquid chromatography-mass spectrometry (LC-MS). In a second approach, a whole-phage shotgun analysis (WSA) was applied. WSA uses trypsin digestion of whole phage particles, followed by reversed-phase HPLC and gas-phase fractionation of the complex peptide mixture prior to MS. The results yield a comprehensive view of structure-related proteins in ϕKMV and suggest subtle structural differences from phage T7.
- Subjects :
- chemistry.chemical_classification
Proteome
biology
Pseudomonas aeruginosa
Shotgun
Peptide
Genome, Viral
Fractionation
medicine.disease_cause
Tandem mass spectrometry
biology.organism_classification
Microbiology
Mass Spectrometry
Bacteriophage
Viral Proteins
chemistry
Lytic cycle
Bacteriophage T7
medicine
Bacteriophages
Chromatography, High Pressure Liquid
Subjects
Details
- ISSN :
- 14652080 and 13500872
- Volume :
- 152
- Database :
- OpenAIRE
- Journal :
- Microbiology
- Accession number :
- edsair.doi.dedup.....17da4623d50e599e1ca34759a66066ec
- Full Text :
- https://doi.org/10.1099/mic.0.28431-0