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Isolation and characterization of a murine resident liver stem cell
- Source :
- Cell death and differentiation. 15(1)
- Publication Year :
- 2007
-
Abstract
- Increasing evidence provides support that mammalian liver contains stem/progenitor cells, but their molecular phenotype, embryological derivation, biology and their role in liver cell turnover and regeneration remain to be further clarified. In this study, we report the isolation, characterization and reproducible establishment in line of a resident liver stem cell (RLSC) with immunophenotype and differentiative potentiality distinct from other previously described liver precursor/stem cells. RLSCs, derived from fetal and neonatal murine livers as well as from immortalized hepatocytic MMH lines and established in lines, are Sca+, CD34-, CD45-, alpha-fetoprotein+ and albumin-. This molecular phenotype suggests a non-hematopoietic origin. RLSC transcriptional profile, defined by microArray technology, highlighted the expression of a broad spectrum of 'plasticity-related genes' and 'developmental genes' suggesting a multi-differentiative potentiality. Indeed, RLSCs spontaneously differentiate into hepatocytes and cholangiocytes and, when cultured in appropriate conditions, into mesenchymal and neuro-ectodermal cell lineages such as osteoblasts/osteocytes, chondrocytes, astrocytes and neural cells. RLSC capability to spontaneously differentiate into hepatocytes, the lack of albumin expression and the broad differentiative potentiality locate them in a pre-hepatoblast/liver precursor cells hierarchical position. In conclusion, RLSCs may provide a useful tool to improve liver stem cell knowledge and to assess new therapeutic approaches for liver diseases.
- Subjects :
- Cellular differentiation
Liver Stem Cell
Cell Separation
Biology
Immunophenotyping
Liver progenitor cells
Mice
Chondrocytes
hepatocyte
Animals
Cell Lineage
Progenitor cell
differentiation
Molecular Biology
Cells, Cultured
Multipotent Stem Cell
Oligonucleotide Array Sequence Analysis
Neurons
Osteoblasts
Animal
Oligonucleotide Array Sequence Analysi
Liver cell
Osteoblast
Gene Expression Profiling
Multipotent Stem Cells
Mesenchymal stem cell
Cell Differentiation
Cell Biology
Neuron
Chondrocyte
Molecular biology
Liver regeneration
Cell biology
Phenotype
Animals, Newborn
Liver
Hepatocytes
Stem cell
Subjects
Details
- ISSN :
- 13509047
- Volume :
- 15
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Cell death and differentiation
- Accession number :
- edsair.doi.dedup.....17cfbe15bdb7853037f9bb3e67e9efbe