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Toxicity of Amorphigenin from the Seeds of Amorpha fruticosa against the Larvae of Culex pipiens pallens (Diptera: Culicidae)
- Source :
- Molecules, Molecules, Vol 20, Iss 2, Pp 3238-3254 (2015)
- Publication Year :
- 2015
- Publisher :
- MDPI AG, 2015.
-
Abstract
- The larvicidal activity of the crude petroleum ether, ethyl acetate, acetone, chloroform and ethanol extracts of Amorpha fruticosa seeds was individually assayed for toxicity against the early fourth-instar larva of the mosquito, Culex pipiens pallens after 24 h exposure. Of the tested extracts, the ethanol one exhibited the highest larvicidal activity (LC50 = 22.69 mg/L). Amorphigenin (8'-hydroxyrotenone), a rotenoid compound which exhibits a strong larvicidal activity with LC50 and LC90 values of 4.29 and 11.27 mg/L, respectively, was isolated from the ethanol extract by column chromatograpy. Its structure was elucidated by 1H-NMR, UV and IR spectral data. Furthermore, investigation of amorphigenin’s effects on mitochondrial complex I activity and protein synthesis in C. pipiens pallens larvae reveals that amorphigenin decreases mitochondrial complex I activities to 65.73% at 10.45 μmol/L, compared to the control, when NADH were used as the substrate. Meanwhile, amorphigenin at 10.45 μmol/L also caused a 1.98-fold decrease in protein content, compared to the control larvae treated with acetone only.
- Subjects :
- larvicidal activity
Insecticides
protein synthesis
Ethyl acetate
Pharmaceutical Science
Rotenoid
Analytical Chemistry
lcsh:QD241-441
chemistry.chemical_compound
Culex pipiens pallens
lcsh:Organic chemistry
Rotenone
Drug Discovery
Botany
Acetone
Animals
Petroleum ether
Food science
amorphigenin
Physical and Theoretical Chemistry
Larva
Ethanol
Chloroform
biology
Communication
Organic Chemistry
Fabaceae
biology.organism_classification
Culex
chemistry
Chemistry (miscellaneous)
Seeds
Amorpha fruticosa
Molecular Medicine
mitochondrial complex I
Subjects
Details
- ISSN :
- 14203049
- Volume :
- 20
- Database :
- OpenAIRE
- Journal :
- Molecules
- Accession number :
- edsair.doi.dedup.....17bca5d8f4219a938a0eada0dd74bc46
- Full Text :
- https://doi.org/10.3390/molecules20023238