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Toxicity of Amorphigenin from the Seeds of Amorpha fruticosa against the Larvae of Culex pipiens pallens (Diptera: Culicidae)

Authors :
Mingshan Ji
Peiwen Qin
Yaping Liang
Zumin Gu
Xiuwei Li
Source :
Molecules, Molecules, Vol 20, Iss 2, Pp 3238-3254 (2015)
Publication Year :
2015
Publisher :
MDPI AG, 2015.

Abstract

The larvicidal activity of the crude petroleum ether, ethyl acetate, acetone, chloroform and ethanol extracts of Amorpha fruticosa seeds was individually assayed for toxicity against the early fourth-instar larva of the mosquito, Culex pipiens pallens after 24 h exposure. Of the tested extracts, the ethanol one exhibited the highest larvicidal activity (LC50 = 22.69 mg/L). Amorphigenin (8'-hydroxyrotenone), a rotenoid compound which exhibits a strong larvicidal activity with LC50 and LC90 values of 4.29 and 11.27 mg/L, respectively, was isolated from the ethanol extract by column chromatograpy. Its structure was elucidated by 1H-NMR, UV and IR spectral data. Furthermore, investigation of amorphigenin’s effects on mitochondrial complex I activity and protein synthesis in C. pipiens pallens larvae reveals that amorphigenin decreases mitochondrial complex I activities to 65.73% at 10.45 μmol/L, compared to the control, when NADH were used as the substrate. Meanwhile, amorphigenin at 10.45 μmol/L also caused a 1.98-fold decrease in protein content, compared to the control larvae treated with acetone only.

Details

ISSN :
14203049
Volume :
20
Database :
OpenAIRE
Journal :
Molecules
Accession number :
edsair.doi.dedup.....17bca5d8f4219a938a0eada0dd74bc46
Full Text :
https://doi.org/10.3390/molecules20023238