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Trinucleotide phosphoramidites: ideal reagents for the synthesis of mixed oligonucleotides for random mutagenesis
- Source :
- ResearcherID
- Publication Year :
- 1994
- Publisher :
- Oxford University Press (OUP), 1994.
-
Abstract
- Trinucleotide phosphoramidites representing codons for all 20 amino acids have been prepared and used in automated, solid-phase DNA synthesis. In contrast to an earlier report, we show that these substances can be used to introduce entire codons into oligonucleotides in excess of 98% yield, and are ideal reagents for the synthesis of mixed oligonucleotides for random mutagenesis.
- Subjects :
- chemistry.chemical_classification
Magnetic Resonance Spectroscopy
Ideal (set theory)
Base Sequence
DNA synthesis
Oligonucleotide
Molecular Sequence Data
Mutagenesis
Nuclear magnetic resonance spectroscopy
Biology
Combinatorial chemistry
Amino acid
Oligodeoxyribonucleotides
Biochemistry
chemistry
Reagent
Yield (chemistry)
Genetics
Codon
Subjects
Details
- ISSN :
- 13624962 and 03051048
- Volume :
- 22
- Database :
- OpenAIRE
- Journal :
- Nucleic Acids Research
- Accession number :
- edsair.doi.dedup.....17bc2011bf26f21de81f2308c4a4ba55