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Collocation of avian and mammal antibodies to develop a rapid and sensitive diagnostic tool for Russell's Vipers Snakebite
- Source :
- PLoS Neglected Tropical Diseases, PLoS Neglected Tropical Diseases, Vol 14, Iss 9, p e0008701 (2020)
- Publication Year :
- 2020
- Publisher :
- Public Library of Science, 2020.
-
Abstract
- Russell’s vipers (RVs) envenoming is an important public health issue in South-East Asia. Disseminated intravascular coagulopathy, systemic bleeding, hemolysis, and acute renal injury are obvious problems that develop in most cases, and neuromuscular junction blocks are an additional problem caused by western RV snakebite. The complex presentations usually are an obstacle to early diagnosis and antivenom administration. Here, we tried to produce highly specific antibodies in goose yolks for use in a paper-based microfluidic diagnostic kit, immunochromatographic test of viper (ICT-Viper), to distinguish RVs from other vipers and even cobra snakebite in Asia. We used indirect ELISA to monitor specific goose IgY production and western blotting to illustrate the interaction of avian or mammal antibody with venom proteins. The ICT-Viper was tested not only in prepared samples but also in stored patient serum to demonstrate its preliminary efficacy. The results revealed that specific anti-Daboia russelii IgY could be raised in goose eggs effectively without inducing adverse effects. When it was collocated with horse anti-Daboia siamensis antibody, which broadly reacted with most of the venom proteins of both types of Russell’s viper, the false cross-reactivity was reduced, and the test showed good performance. The limit of detection was reduced to 10 ng/ml in vitro, and the test showed good detection ability in clinical snake envenoming case samples. The ICT-Viper performed well and could be combined with a cobra venom detection kit (ICT-Cobra) to create a multiple detection strip (ICT-VC), which broadens its applications while maintaining its detection ability for snake envenomation identification. Nonetheless, the use of the ICT-Viper in the South-East Asia region is pending additional laboratory and field investigations and regional collaboration. We believe that the development of this practical diagnostic tool marks the beginning of positive efforts to face the global snakebite issue.<br />Author summary For a long time, many scientists have made efforts to develop more effective antivenoms or to expand the limits of existing antivenoms. Such endeavors are challenging but worth the effort in the long run. However, to use precious antivenom more effectively, a snake species discrimination tool might be helpful for treatment by ensuring accurate antivenom usage and dosage. In this study, we revealed that avian IgY might be an economical alternative for collocation with the equine F(ab’)2 fragment in the development of a diagnostic device with improved performance. Only a few avian eggs (about 10–15) would be necessary to produce 10,000 kits. Immunochromatographic test (ICT) is a simple, easy, fast, and low-cost point-of-care lateral flow assay device, and especially adapted to perform in resource-deficient areas. Such an antibody collocation strategy might be beneficial to overcome the urgent need for snakebite diagnosis as soon as possible. More cooperative investigations across Asia continent might be initiated in the future to face the challenge of snakebites together.
- Subjects :
- Physiology
Antivenom
RC955-962
Snake Bites
Cobra
Venom
Toxicology
Pathology and Laboratory Medicine
Biochemistry
Medical Conditions
Arctic medicine. Tropical medicine
Immune Physiology
Geese
Medicine and Health Sciences
Medicine
Toxins
Snakebite
Enzyme-Linked Immunoassays
Asia, Southeastern
computer.programming_language
Mammals
Immune System Proteins
biology
Elapid Venoms
Antivenins
Eukaryota
Snakes
Acute Kidney Injury
Squamates
Infectious Diseases
Vertebrates
Russell's Viper
Antibody
Public aspects of medicine
RA1-1270
Research Article
Neglected Tropical Diseases
VIPeR
Asia
Toxic Agents
Immunology
Equines
Vipers
Immunoglobulins
Enzyme-Linked Immunosorbent Assay
Hemorrhage
Research and Analysis Methods
Antibodies
Birds
Goose
biology.animal
Animals
Humans
Horses
Immunoassays
business.industry
Venoms
Diagnostic Tests, Routine
Public Health, Environmental and Occupational Health
Organisms
Biology and Life Sciences
Reptiles
Proteins
Tropical Diseases
Amniotes
biology.protein
Immunologic Techniques
business
computer
Zoology
Subjects
Details
- Language :
- English
- ISSN :
- 19352735 and 19352727
- Volume :
- 14
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- PLoS Neglected Tropical Diseases
- Accession number :
- edsair.doi.dedup.....17b27c309993544da934f8047291fc67