Back to Search
Start Over
Expression of PITX2 homeodomain transcription factor during rat gonadal development in a sexually dimorphic manner
- Source :
- Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology. 27(2)
- Publication Year :
- 2011
-
Abstract
- PITX2, a multifunctional Paired-like homeodomain transcription factor, plays obligatory role during development of organs like heart, brain and pituitary. It regulates differentiation of vascular smooth muscle cells and hematopoietic stem cells. Although we earlier reported the Pitx2/PITX2 expression in gonad, but the expression pattern of its different isoforms in mammalian gonads especially during development is still not known. As PITX2 participates in the development of multiple organs and different homeobox genes have been shown to control gonadal functions, we wanted to investigate the role of PITX2 in gonadal development and its function. The objective of our study was to know the expression profile of different Pitx2/PITX2 isoforms and its localization throughout the development of gonads. Here we show the temporal and spatial expression pattern of Pitx2/PITX2 and its localization throughout the embryonic and postnatal stages of rat gonads. Pitx2/PITX2 expression profile reveals the differential and dimorphic expression pattern of its two isoforms PITX2B2 and-Cβ throughout the embryonic development stages and also in the postnatal stages, where it becomes more prominent. This is the first report where PITX2 homeodomain transcription factor shows isoform-specific sexually dimorphic expression. In addition, PITX2 localization was found in the embryonic ovarian primordial germ cell clusters and germ cells inside the testicular cords and also in somatic cells. In adults, ovarian granulosa and theca cells as well as germ cells inside the seminiferous tubules in testis express PITX2. All the evidences suggest that the differential expression of PITX2 might be associated with sex-specific embryonic and postnatal gonadal development and the physiological processes.
- Subjects :
- Male
congenital, hereditary, and neonatal diseases and abnormalities
endocrine system
medicine.medical_specialty
Gonad
Physiology
Somatic cell
Molecular Sequence Data
Embryonic Development
Biology
Rats, Sprague-Dawley
Epitopes
stomatognathic system
Pregnancy
Internal medicine
Testis
medicine
Animals
Protein Isoforms
Amino Acid Sequence
Gonads
Homeodomain Proteins
Sex Characteristics
PITX2
Embryogenesis
Ovary
Embryo, Mammalian
Embryonic stem cell
Cell biology
Rats
stomatognathic diseases
Endocrinology
medicine.anatomical_structure
Homeobox
Female
sense organs
Development of the gonads
Stem cell
Transcription Factors
Subjects
Details
- ISSN :
- 14219778
- Volume :
- 27
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology
- Accession number :
- edsair.doi.dedup.....17adf65e9a221f61e874e095333580f9