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Jasmonate regulates the FAMA/mediator complex subunit 8-THIOGLUCOSIDE GLUCOHYDROLASE 1 cascade and myrosinase activity
- Source :
- Plant Physiol
- Publication Year :
- 2021
- Publisher :
- Oxford University Press, 2021.
-
Abstract
- Myrosinases are β-thioglucoside glucosidases that are unique to the Brassicales order. These enzymes hydrolyze glucosinolates to produce compounds that have direct antibiotic effects or that function as signaling molecules in the plant immune system, protecting plants from pathogens and insect pests. However, the effects of jasmonic acid (JA), a plant hormone that is crucial for plant disease resistance, on myrosinase activity remain unclear. Here, we systematically studied the effects of JA on myrosinase activity and explored the associated internal transcriptional regulation mechanisms. Exogenous application of JA significantly increased myrosinase activity, while the inhibition of endogenous JA biosynthesis and signaling reduced myrosinase activity. In addition, some myrosinase genes in Arabidopsis (Arabidopsis thaliana) were upregulated by JA. Further genetic and biochemical evidence showed that transcription factor FAMA interacted with a series of JASMONATE ZIM-DOMAIN proteins and affected JA-mediated myrosinase activity. However, among the JA-upregulated myrosinase genes, only THIOGLUCOSIDE GLUCOHYDROLASE 1 (TGG1) was positively regulated by FAMA. Further biochemical analysis showed that FAMA bound to the TGG1 promoter to directly mediate TGG1 expression in conjunction with Mediator complex subunit 8 (MED8). Together, our results provide evidence that JA acts as an important signal upstream of the FAMA/MED8–TGG1 pathway to positively regulate myrosinase activity in Arabidopsis.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Regular Issue
Glycoside Hydrolases
Physiology
Arabidopsis
Cyclopentanes
Plant Science
Corrections
01 natural sciences
03 medical and health sciences
chemistry.chemical_compound
Basic Helix-Loop-Helix Transcription Factors
Genetics
Transcriptional regulation
Arabidopsis thaliana
Oxylipins
Jasmonate
Transcription factor
Mediator Complex
biology
Arabidopsis Proteins
Chemistry
Myrosinase
Jasmonic acid
biology.organism_classification
030104 developmental biology
Biochemistry
Plant hormone
Signal Transduction
010606 plant biology & botany
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Plant Physiol
- Accession number :
- edsair.doi.dedup.....1796d02f7c15a7526c8466f3159ffed5