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A liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based assay to profile 20 plasma steroids in endocrine disorders
- Source :
- Clinical Chemistry and Laboratory Medicine (CCLM). 58:1477-1487
- Publication Year :
- 2020
- Publisher :
- Walter de Gruyter GmbH, 2020.
-
Abstract
- Background Liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based assays are employed in more and more clinical laboratories to quantify steroids. The steroid quantification by LC-MS/MS shows great value in screening or diagnosing endocrine disorders; however, the number of functional steroids included in the LC-MS/MS methods is still limited. Methods Here, we describe the performance and validation of a 20-steroid plasma panel by LC-MS/MS. The panel included progestogens (including mineralocorticoids and glucocorticoids), androgens and estrogens biosynthesized in steroid metabolic pathways. The LC-MS/MS method was validated according to guidance documents, and subsequently employed to profile steroid changes in endocrine disorders. Results Using LC-MS/MS, 20 steroids were separated and quantified in 8 min. Coefficients of variation (CVs) of the 20 analytes at the lower limit of quantification (LLoQ) were all less than 15% (ranging from 1.84% to 14.96%). The linearity of the assay was demonstrated by all the R2 values greater than 0.995. Individual plasma steroids changed significantly in patients with subclinical Cushing’s syndrome (SCS) and polycystic ovary syndrome (PCOS) – 17-hydroxypregnenolone (17-OH-PR), testosterone (T) and dihydrotestosterone (DHT) were significantly decreased in SCS patients, while in PCOS patients, pregnenolone, corticosterone (CORT), androstenedione (A4) and T were significantly increased and DHT was decreased. Conclusions The LC-MS/MS method we developed for the quantification of 20 plasma steroids is clinical practicable. The steroid profiling data using this assay indicate its screening value for endocrine disorders. To further explore the value of the assay, more investigations are however needed.
- Subjects :
- medicine.medical_specialty
medicine.medical_treatment
Clinical Biochemistry
030209 endocrinology & metabolism
Sensitivity and Specificity
Steroid
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Limit of Detection
Tandem Mass Spectrometry
Corticosterone
Liquid chromatography–mass spectrometry
Internal medicine
Humans
Medicine
Endocrine system
Androstenedione
Pituitary ACTH Hypersecretion
business.industry
Biochemistry (medical)
Reproducibility of Results
General Medicine
Polycystic ovary
Endocrinology
chemistry
030220 oncology & carcinogenesis
Dihydrotestosterone
Pregnenolone
Female
Steroids
business
Chromatography, Liquid
Polycystic Ovary Syndrome
medicine.drug
Subjects
Details
- ISSN :
- 14374331 and 14346621
- Volume :
- 58
- Database :
- OpenAIRE
- Journal :
- Clinical Chemistry and Laboratory Medicine (CCLM)
- Accession number :
- edsair.doi.dedup.....178b8c33a119a773ae41987783053a58