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The Drosophila DOCK family protein Sponge is required for development of the air sac primordium
- Source :
- Experimental cell research. 354(2)
- Publication Year :
- 2016
-
Abstract
- Dedicator of cytokinesis (DOCK) family genes are known as DOCK1-DOCK11 in mammals. DOCK family proteins mainly regulate actin filament polymerization and/or depolymerization and are GEF proteins, which contribute to cellular signaling events by activating small G proteins. Sponge (Spg) is a Drosophila counterpart to mammalian DOCK3/DOCK4, and plays a role in embryonic central nervous system development, R7 photoreceptor cell differentiation, and adult thorax development. In order to conduct further functional analyses on Spg in vivo, we examined its localization in third instar larval wing imaginal discs. Immunostaining with purified anti-Spg IgG revealed that Spg mainly localized in the air sac primordium (ASP) in wing imaginal discs. Spg is therefore predicted to play an important role in the ASP. The specific knockdown of Spg by the breathless-GAL4 driver in tracheal cells induced lethality accompanied with a defect in ASP development and the induction of apoptosis. The monitoring of ERK signaling activity in wing imaginal discs by immunostaining with anti-diphospho-ERK IgG revealed reductions in the ERK signal cascade in Spg knockdown clones. Furthermore, the overexpression of D-raf suppressed defects in survival and the proliferation of cells in the ASP induced by the knockdown of Spg. Collectively, these results indicate that Spg plays a critical role in ASP development and tracheal cell viability that is mediated by the ERK signaling pathway.
- Subjects :
- 0301 basic medicine
MAPK/ERK pathway
Cell signaling
Cytoplasm
animal structures
MAP Kinase Signaling System
Apoptosis
Biology
03 medical and health sciences
Animals
Drosophila Proteins
Actin filament polymerization
Extracellular Signal-Regulated MAP Kinases
Air Sacs
Photoreceptor cell differentiation
Dock3
Cell Membrane
Dock4
Cell Biology
Cell biology
Trachea
Imaginal disc
030104 developmental biology
Drosophila melanogaster
Biochemistry
Gene Knockdown Techniques
Carrier Proteins
Cytokinesis
Subjects
Details
- ISSN :
- 10902422
- Volume :
- 354
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Experimental cell research
- Accession number :
- edsair.doi.dedup.....178b12889e097b90869f3c2b54ed0b00