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Monitoring seasonal influenza A evolution: rapid 2009 pandemic H1N1 surveillance with an reverse transcription-polymerase chain reaction/electro-spray ionization mass spectrometry assay
- Source :
- Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology. 54(4)
- Publication Year :
- 2012
-
Abstract
- Background The emergence of the pandemic H1N1 influenza strain in 2009 reinforced the need for improved influenza surveillance efforts. A previously described influenza typing assay that utilizes RT-PCR coupled to electro-spray ionization mass spectrometry (ESI-MS) played an early role in the discovery of the pandemic H1N1 influenza strain, and has potential application for monitoring viral genetic diversity in ongoing influenza surveillance efforts. Objectives To determine the analytical sensitivity of RT-PCR/ESI-MS influenza typing assay for identifying the pandemic H1N1 strain and describe its ability to assess viral genetic diversity. Study design Two sets of pandemic H1N1 samples, 190 collected between April and June of 2009, and 69 collected between October 2009 and January 2010, were processed by the RT-PCR/ESI-MS influenza typing assay, and the spectral results were compared to reference laboratory results and historical sequencing data from the Nucleotide Database of the National Center for Biotechnology Information (NCBI). Results Strain typing concordance with reference standard testing was 100% in both sample sets, and the assay demonstrated a significant increase in influenza genetic diversity, from 10.5% non-wildtype genotypes in early samples to 69.9% in late samples (P
- Subjects :
- Spectrometry, Mass, Electrospray Ionization
Time Factors
Biology
medicine.disease_cause
Sensitivity and Specificity
Virus
Article
Evolution, Molecular
Influenza A Virus, H1N1 Subtype
Virology
Pandemic
Genotype
Influenza, Human
Influenza A virus
medicine
Humans
Typing
Genotyping
Pandemics
Genetic diversity
Reverse Transcriptase Polymerase Chain Reaction
Strain (biology)
virus diseases
Infectious Diseases
Population Surveillance
Seasons
Subjects
Details
- ISSN :
- 18735967
- Volume :
- 54
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology
- Accession number :
- edsair.doi.dedup.....178a7e87e9e2b469e8a5b1ffc262216f