Back to Search
Start Over
Production and development of porcine tetraploid parthenogenetic embryos
- Source :
- Journal of Animal Science and Technology, Journal of Animal Science and Technology, Vol 61, Iss 4, Pp 225-233 (2019)
- Publication Year :
- 2019
- Publisher :
- Korean Society of Animal Sciences and Technology, 2019.
-
Abstract
- The aim of this study was to produce porcine tetraploid (4N) parthenogenetic embryos using various methods and evaluate their developmental potential. In method 1 (M1), porcine 4N parthenogenetic embryos were obtained by inhibiting extrusion of both first (PB1) and second (PB2) polar bodies; in methods 2 (M2) and 3 (M3), 4N parthenogenetic embryos were obtained by electrofusion of 2-cell stage diploid parthenogenetic embryos derived from inhibition of PB2 or PB1 extrusion, respectively. We found no differences in the rates of cleavage or blastocyst formation or the proportion of 4N embryos among M1, M2, and M3 groups. The different methods also did not influence apoptosis rates (number of TUNEL-positive cells/number of total cells) or expression levels of apoptosis-related BAX and BCL2L1 genes. However, total cell and EdU (5-ethynyl-2’-deoxyuridine)-positive cell numbers in 4N parthenogenetic blastocysts derived from M1 were higher (p < 0.05) than those for M2 and M3 groups. Our results suggest that, although porcine 4N parthenogenetic embryos could be produced by a variety of methods, inhibition of PB1 and PB2 extrusion (M1) is superior to electrofusion of 2-cell stage diploid parthenogenetic embryos derived from inhibition of PB2 (M2) or PB1 (M3) extrusion.
- Subjects :
- 0301 basic medicine
animal structures
Veterinary (miscellaneous)
viruses
Cell
Parthenogenesis
Biology
Cleavage (embryo)
Biochemistry, Genetics and Molecular Biology (miscellaneous)
Electrofusion
Andrology
03 medical and health sciences
Polar body
medicine
Blastocyst
lcsh:SF1-1100
Proliferatio
Ecology
0402 animal and dairy science
virus diseases
Embryo
04 agricultural and veterinary sciences
biochemical phenomena, metabolism, and nutrition
040201 dairy & animal science
030104 developmental biology
medicine.anatomical_structure
Porcine tetraploid embryo
embryonic structures
Animal Science and Zoology
lcsh:Animal culture
Ploidy
Food Science
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 20550391
- Volume :
- 61
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Journal of Animal Science and Technology
- Accession number :
- edsair.doi.dedup.....178329b6f2e1a8c8a3dd61b7dbc1a2e2