Supplementary Figures 1-8 from Hyperthermia Synergizes with Chemotherapy by Inhibiting PARP1-Dependent DNA Replication Arrest

Supplemental Figures (Supplementary Fig. 1-8): Cell lines are HR proficient (Supplementary Fig. 1); Influence of hyperthermia on the quantity of intra-nuclear Pt-DNA adducts and intracellular doxorubicin concentration (Supplementary Fig. 2); Hyperthermia sensitizes tumor cells to drugs which induce PARylation without altering PARP of PARG protein levels (Supplementary Fig. 3); Hyperthermia leads to a delayed DNA strand break repair capacity in different cell lines from ovarian and colon cancer (Supplementary Fig. 4); HR competence in 14 of 15 samples from PC patients (Supplementary Fig. 5); Gamma-irradiation leads to induction of γH2AX foci in all cells independent of proliferation status (Supplementary Fig. 6); Hyperthermia has no acute effect on cisplatin or doxorubicin induced apoptosis (Supplementary Fig. 7); Combined treatment of cells with hyperthermia and PARPi slightly enhanced the potentiating effect of either treatment alone (Supplementary Fig. 8).