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Ankyrin G overexpression in Hutchinson-Gilford progeria syndrome fibroblasts identified through biological filtering of expression profiles
- Source :
- Journal of human genetics. 51(11)
- Publication Year :
- 2006
-
Abstract
- Hutchinson-Gilford progeria syndrome (HGPS; MIM 176670) is a rare disease characterized by accelerated aging. In this study, light and immunofluorescence microscopy were used to assess morphological changes, measures of cell growth kinetics and gene expression profiles in HGPS cells and normal fibroblasts in culture. A filtering strategy was developed based on differentially expressed transcripts seen consistently across three culture stages based on cell passage number. This filtering strategy produced a list of 66 unique differentially expressed genes, of which approximately 40% were upregulated in HGPS cells compared to normal fibroblasts. The increased mRNA expression in HGPS cells that was seen for one gene defined using this strategy--namely ANK3--was validated using quantitative reverse-transcriptase amplification, Western analysis and immunofluorescence microscopy, all of which showed significantly increased ankyrin G expression. These findings demonstrate differences in morphology, growth kinetics and mRNA expression profiles in HGPS cells compared to normal fibroblasts in culture, including increased expression of ANK3/ankyrin G. Furthermore, other genes that co-clustered with ANK3 might provide mechanistic clues regarding senescence in cultured HGPS cells.
- Subjects :
- Senescence
Ankyrins
congenital, hereditary, and neonatal diseases and abnormalities
Biology
Cell Line
Progeria
Gene expression
Genetics
medicine
Ankyrin
Cluster Analysis
Humans
ANK3
Epigenetics
RNA, Messenger
Genetics (clinical)
Cell Proliferation
Oligonucleotide Array Sequence Analysis
chemistry.chemical_classification
Genome
integumentary system
Cell growth
Reverse Transcriptase Polymerase Chain Reaction
nutritional and metabolic diseases
Fibroblasts
medicine.disease
Molecular biology
Kinetics
chemistry
Gene Expression Regulation
Microscopy, Fluorescence
Cell culture
Subjects
Details
- ISSN :
- 14345161
- Volume :
- 51
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- Journal of human genetics
- Accession number :
- edsair.doi.dedup.....1767a8d7f73209eb0b827d8a7d641b1e