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The α-anomeric form of sialic acid is the minimal receptor determinant recognized by reovirus
- Source :
- Virology. 172:382-385
- Publication Year :
- 1989
- Publisher :
- Elsevier BV, 1989.
-
Abstract
- A series of synthetic sialosides were evaluated for their ability to interact with reovirus serotype 3. It was found that sialosides with terminal N-acetylneuraminic acid (NeuNAc) linked in either an alpha 2,3 or alpha 2,6 configuration effectively blocked the binding of reovirus to mouse L fibroblasts, in contrast to a monosaccharide mixture containing the oligosaccharide constituents. Direct binding of reovirus to the sialosides was also demonstrable using sialosides conjugated to bovine serum albumin as ligands in a solid phase binding system. Of particular significance was the finding that the conjugate containing alpha-sialic acid alone (linked to bovine serum albumin) was capable of being recognized by reovirus at a level comparable to that of the other sialoside conjugates. Virus binding was abrogated by pretreating such conjugates with neuraminidase. These results suggest that the alpha-anomeric form of sialic acid is the minimal receptor determinant for reovirus recognition.
- Subjects :
- chemistry.chemical_classification
Anomer
Molecular Structure
biology
Sialoglycoproteins
viruses
Molecular Sequence Data
Reoviridae
Oligosaccharide
biology.organism_classification
Sialic acid
Structure-Activity Relationship
chemistry.chemical_compound
Carbohydrate Sequence
chemistry
Biochemistry
Virology
Sialic Acids
biology.protein
Receptors, Virus
Bovine serum albumin
Receptor
Neuraminidase
Conjugate
Subjects
Details
- ISSN :
- 00426822
- Volume :
- 172
- Database :
- OpenAIRE
- Journal :
- Virology
- Accession number :
- edsair.doi.dedup.....17647dea58938430b506e7ddf730b0cb