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Expression of BARD1 β Isoform in Selected Pediatric Tumors
- Source :
- Genes, Volume 12, Issue 2, Genes, Vol 12, Iss 168, p 168 (2021)
- Publication Year :
- 2021
- Publisher :
- MDPI AG, 2021.
-
Abstract
- Currently, many new possible biomarkers and mechanisms are being searched and tested to analyse pathobiology of pediatric tumours for the development of new treatments. One such candidate molecular factor is BARD1 (BRCA1 Associated RING Domain 1)—a tumour-suppressing gene involved in cell cycle control and genome stability, engaged in several types of adult-type tumours. The data on BARD1 significance in childhood cancer is limited. This study determines the expression level of BARD1 and its isoform beta (β) in three different histogenetic groups of pediatric cancer – neuroblastic tumours, and for the first time in chosen germ cell tumours (GCT), and rhabdomyosarcoma (RMS), using the qPCR method. We found higher expression of beta isoform in tumour compared to healthy tissue with no such changes concerning BARD1 full-length. Additionally, differences in expression of BARD1 β between histological types of neuroblastic tumours were observed, with higher levels in ganglioneuroblastoma and ganglioneuroma. Furthermore, a higher expression of BARD1 β characterized yolk sac tumours (GCT type) and RMS when comparing with non-neoplastic tissue. These tumours also showed a high expression of the TERT (Telomerase Reverse Transcriptase) gene. In two RMS cases we found deep decrease of BARD1 β in post-chemotherapy samples. This work supports the oncogenicity of the beta isoform in pediatric tumours, as well as demonstrates the differences in its expression depending on the histological type of neoplasm, and the level of maturation in neuroblastic tumours.
- Subjects :
- Male
0301 basic medicine
0302 clinical medicine
Neoplasms
Protein Isoforms
Neoplasm
germ cell tumors
Child
Rhabdomyosarcoma
Genetics (clinical)
Ganglioneuroblastoma
Age Factors
Exons
Gene Expression Regulation, Neoplastic
Organ Specificity
Child, Preschool
030220 oncology & carcinogenesis
Female
Gene isoform
lcsh:QH426-470
Ubiquitin-Protein Ligases
TERT
Biology
Article
splicing
neuroblastoma
03 medical and health sciences
Neuroblastoma
Biomarkers, Tumor
Genetics
medicine
Humans
BARD1
BARD1 isoforms
Telomerase reverse transcriptase
Ganglioneuroma
yolk sac tumors
pediatric tumor
Neoplasm Staging
Tumor Suppressor Proteins
Infant
medicine.disease
Alternative Splicing
lcsh:Genetics
BARD1 β
030104 developmental biology
Cancer research
rhabdomyosarcoma
Germ cell tumors
Neoplasm Grading
Subjects
Details
- ISSN :
- 20734425
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Genes
- Accession number :
- edsair.doi.dedup.....1762bf0b75920e2ac9a8c32915db936f