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Characterization of Small Plaque Mutants of Mouse Hepatitis Virus, JHM Strain
Characterization of Small Plaque Mutants of Mouse Hepatitis Virus, JHM Strain
- Source :
- Microbiology and Immunology
- Publication Year :
- 2013
- Publisher :
- John Wiley and Sons Inc., 2013.
-
Abstract
- Two small plaque mutants designated as 1a and 2c were isolated from DBT cells persistently infected with the JHM strain of mouse hepatitis virus. Unlike the wild type JHM, these two mutant viruses grew more slowly with no prominent cell fusion. The buoyant densities of the mutants were slightly lower and 2c was revealed to have fewer peplomers than JHM by electron microscopy. The purified JHM contained five polypeptides with molecular weights (M.W.) of 260,000, 105,000 (GP105), 65,000, 60,000 (P60), and 23,000 (GP23). In addition to two polypeptides, P60 and GP23, which were common to JHM and the mutants, 1a was found to contain three other specific polypeptides with M.W. of 180,000 (GP160), 110,000, and 95,000 (GP95), while 2c had GP180, GP105, GP95, and one with a M.W. of 175,000. All of these polypeptides were shown to be glycosylated except for P60. After bromelain treatment, all these viruses lost the peplomers and contained P60 and another new 18,000 dalton polypeptide.
- Subjects :
- Viral Plaque Assay
Genes, Viral
viruses
Immunology
Mutant
Microbiology
Virus
Cell Line
Mice
Viral Proteins
Mouse hepatitis virus
Cytopathogenic Effect, Viral
Virology
Centrifugation, Density Gradient
Animals
Murine hepatitis virus
Cell fusion
biology
Molecular mass
Wild type
Glycopeptides
Temperature
virus diseases
Original Articles
biology.organism_classification
Bromelains
Molecular Weight
Cell culture
Mutation
Original Article
Subjects
Details
- Language :
- English
- ISSN :
- 13480421 and 03855600
- Volume :
- 27
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Microbiology and Immunology
- Accession number :
- edsair.doi.dedup.....1756bfbd6fdc6b959fd0f348f07a28cd